Morlon-Guyot, J., Berry, L., Sauquet, I., Singh Pall, G., El Hajj, H., Meissner, M. and Daher, W. (2018) Conditional knock-down of a novel coccidian protein leads to the formation of aberrant apical organelles and abrogates mature rhoptry positioning in Toxoplasma gondii. Molecular and Biochemical Parasitology, 223, pp. 19-30. (doi: 10.1016/j.molbiopara.2018.06.003) (PMID:29958928)
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Abstract
Toxoplasma gondii which is a member of the coccidian parasites owns a spatially polarized secretory system, which synthesizes de novo micronemes and rhoptries. These apical secretory organelles discharge their contents into host cells promoting invasion and survival. Herein, we identified a novel Coccidian Specific CORVET/HOPS Associated Protein (CSCHAP) belonging to the interaction network of both tethering complexes. CSCHAP is associated with the endomembrane system, rhoptries, micronemes and probably to the inner core of the conoid. Conditional depletion of CSCHAP leads to apical disconnection of rhoptries, aberrant apical organelles biogenesis and severely hinders T. gondii invasion. Overall, our study provides new insights into the mechanisms underpinning secretory organelles biogenesis in coccidian parasites.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was made possible through core support from the Fondation pour la Recherche Médicale (Equipe FRMDEQ20130326508) and the Labex Parafrap (ANR-11-LABX-0024). MM was supported by an ERC Starting grant, (ERC-2012-StG 309255), and a Wellcome Senior Fellowship, 087582/Z/08/Z. |
| Keywords: | CORVET and HOPS, Toxoplasma gondiirhoptry positioning, invasion, microneme biogenesis. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Meissner, Professor Markus and Pall, Dr Gurman |
| Authors: | Morlon-Guyot, J., Berry, L., Sauquet, I., Singh Pall, G., El Hajj, H., Meissner, M., and Daher, W. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Molecular and Biochemical Parasitology |
| Publisher: | Elsevier |
| ISSN: | 0166-6851 |
| ISSN (Online): | 1872-9428 |
| Published Online: | 27 June 2018 |
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