Removing physiological motion from intravital and clinical functional imaging data

Warren, S. C. et al. (2018) Removing physiological motion from intravital and clinical functional imaging data. eLife, 7, e35800. (doi: 10.7554/elife.35800) (PMID:29985127) (PMCID:PMC6037484)

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Intravital microscopy can provide unique insights into the function of biological processes in a native context. However, physiological motion caused by peristalsis, respiration and the heartbeat can present a significant challenge, particularly for functional readouts such as fluorescence lifetime imaging (FLIM), which require longer acquisition times to obtain a quantitative readout. Here, we present and benchmark Galene, a versatile multi-platform software tool for image-based correction of sample motion blurring in both time resolved and conventional laser scanning fluorescence microscopy data in two and three dimensions. We show that Galene is able to resolve intravital FLIM-FRET images of intra-abdominal organs in murine models and NADH autofluorescence of human dermal tissue imaging subject to a wide range of physiological motions. Thus, Galene can enable FLIM imaging in situations where a stable imaging platform is not always possible and rescue previously discarded quantitative imaging data.

Item Type:Articles
Keywords:Tools and resources, cell biology, computational and systems biology, FLIM, FRET, motion correction, intravital microscopy, multiphoton, human, mouse.
Glasgow Author(s) Enlighten ID:Anderson, Professor Kurt and Morton, Professor Jen and Strathdee, Mr Douglas
Creator Roles:
Morton, J.Resources, Writing – review and editing
Strathdee, D.Resources, Writing – review and editing
Anderson, K.Resources, Writing – review and editing
Authors: Warren, S. C., Nobis, M., Magenau, A., Mohammed, Y. H., Herrmann, D., Moran, I., Vennin, C., Conway, J. R.W., Mélénec, P., Cox, T. R., Wang, Y., Morton, J. P., Welch, H. C.E., Strathdee, D., Anderson, K. I., Phan, T. G., Roberts, M. S., and Timpson, P.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:eLife
Publisher:eLife Sciences Publications
ISSN (Online):2050-084X
Copyright Holders:Copyright © 2018 Warren et al.
First Published:First published in eLife 7:e35800
Publisher Policy:Reproduced under a Creative Commons License

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