ISCEV extended protocol for the dark-adapted red flash ERG

Thompson, D. A., Fujinami, K., Perlman, I., Hamilton, R. and Robson, A. G. (2018) ISCEV extended protocol for the dark-adapted red flash ERG. Documenta Ophthalmologica, 136(3), pp. 191-197. (doi: 10.1007/s10633-018-9644-z) (PMID:29934801)

Abstract

The International Society for Clinical Electrophysiology of Vision (ISCEV) standard for full-field electroretinography (ERG) describes a minimum procedure, but encourages more extensive testing. This ISCEV extended protocol describes an extension to the ERG standard, namely the dark-adapted (DA) red flash ERG. The DA red flash ERG can be incorporated conveniently within the ISCEV standard ERG protocol after a minimum of 20-min DA and recorded after the DA 0.01 ERG to a flash strength of 0.3 phot cd s m−2, eliciting a waveform with two positive peaks in healthy individuals. The first positive component is the cone-mediated x-wave with a peak at 30–50 ms; the second is a rod-mediated b-wave with a peak time of approximately 100 ms. Shorter DA times may be desirable to shorten the recording time or to alter the prominence of the early cone-mediated x-wave relative to the rod-mediated b-wave. The DA red flash ERG is used to aid the diagnosis of achromatopsia (rod monochromacy), cone dystrophy and other forms of cone system dysfunction, including “Bradyopsia” (RGS9/R9AP-retinopathy), when the DA red flash ERG x-wave is preserved in the absence of ISCEV standard LA ERGs. The DA red flash ERG can also help determine the origin of residual DA ERGs in cases of severe rod dysfunction, for example in disorders such as vitamin A deficiency, fundus albipunctatus (RDH5-retinopathy), Oguchi disease (SAG- or GRK1-retinopathy) and some rod-cone dystrophies. To shorter DA periods, the x-wave may be elicited without the following rod b-wave, shown to be helpful in abbreviated protocols for children.

• Item Type: Articles
• Keywords: ISCEV standards, clinical standards, electroretinogram (ERG), full-field ERG, International Society of Clinical Electrophysiology of Vision (ISCEV), Dark-Adapted (DA), red flash ERG, retinal dystrophy.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Hamilton, Dr Ruth
• Authors: Thompson, D. A., Fujinami, K., Perlman, I., Hamilton, R., and Robson, A. G.
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
• Journal Name: Documenta Ophthalmologica
• Publisher: Springer
• ISSN: 0012-4486
• ISSN (Online): 1573-2622
• Published Online: 22 June 2018
• Copyright Holders: Copyright © 2018 The Authors
• First Published: First published in Documenta Ophthalmologica 136(3):191-197
• Publisher Policy: Reproduced under a Creative Commons License