Busin, V. , Burgess, S. and Shu, W. (2018) A hybrid paper-based microfluidic platform toward veterinary P-ELISA. Sensors and Actuators B: Chemical, 273, pp. 536-542. (doi: 10.1016/j.snb.2018.06.075)
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Abstract
This study describes a novel fabrication method to produce a hybrid paper-based microfluidic platform, termed Multi-Pad Paper Plate (MP3) for transfer and optimisation of enzyme-linked immunoassays (ELISA) on paper (P-ELISA). The new fabrication technique is based on a combination of laser micromachining of paper and packaging through thermal lamination. This method is simple, rapid and of high-resolution and can produce robust, versatile and low-cost devices, compatible with a standard 96-well microplate format. The MP3 was used to transfer a sandwich ELISA for detection of bovine haptoglobin (Hp), a marker of inflammation in animals allowing optimisation of the assay in the new format. Using the MP3, a standard curve was generated and the limit of detection achieved was 0.73 μg/ml. The optimised protocol was also applied to the detection of Hp in bovine serum, demonstrating the possibility of using this platform with biological samples. The new platform allowed for a reduction of 93% in time and of 88% in cost for performing the assay and represents a valid alternative to other commonly used device fabrication methods, especially in the context of low-resource settings. It has the potential to translate similar assays into P-ELISA and offers a starting point for achieving animal-side testing.
| Item Type: | Articles |
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| Additional Information: | The authors would like to thank Moredun Scientific Ltd. and Heriot-Watt University for funding this research. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Busin, Valentina |
| Authors: | Busin, V., Burgess, S., and Shu, W. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Sensors and Actuators B: Chemical |
| Publisher: | Elsevier |
| ISSN: | 0925-4005 |
| ISSN (Online): | 1873-3077 |
| Published Online: | 18 June 2018 |
| Copyright Holders: | Copyright © 2018 Elsevier B.V. |
| First Published: | First published in Sensors and Actuators B: Chemical 273: 536-542 |
| Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
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