Costa, E., González-García, C., Gómez Ribelles, J. L. and Salmeron-Sanchez, M. (2018) Maintenance of chondrocyte phenotype during expansion on PLLA microtopographies. Journal of Tissue Engineering, 9, pp. 1-10. (doi: 10.1177/2041731418789829) (PMID:30093985) (PMCID:PMC6080075)
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Abstract
Articular chondrocytes are difficult to grow as they lose their characteristic phenotype following expansion on standard tissue culture plates. Here we show that culturing them on surfaces of poly(L-lactic acid), PLLA, of well-defined microtopography allows expansion and maintenance of characteristic chondrogenic markers. We investigated the dynamics of human chondrocyte dedifferentiation on the different PLLA microtopograhies by the expression of collagen type I, collagen type II and aggrecan at different culture times. When seeded on PLLA, chondrocytes maintained their characteristic hyaline phenotype up to 7 days, which allowed expand the initial cell population approximately six times without cell dedifferentiation. Maintenance of cell phenotype was afterwards correlated to cell adhesion on the different substrates. Chondrocytes adhesion occurs via the a5b1 integrin on PLLA, suggesting cellfibronectin interactions. However, a2b1 integrin is mainly expressed on the control substrate after 1 day of culture and the characteristic chondrocytic markers are lost (collagen type II expression is overcome by the synthesis of collagen type I). Expanding chondrocytes on PLLA might be an effective solution to prevent dedifferentiation and improving the number of cells needed for autologous chondrocyte transplantation.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Salmeron-Sanchez, Professor Manuel |
Authors: | Costa, E., González-García, C., Gómez Ribelles, J. L., and Salmeron-Sanchez, M. |
College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
Journal Name: | Journal of Tissue Engineering |
Publisher: | SAGE |
ISSN: | 2041-7314 |
ISSN (Online): | 2041-7314 |
Copyright Holders: | Copyright © 2018 The Authors |
First Published: | First published in Journal of Tissue Engineering 9: 2041731418789829 |
Publisher Policy: | Reproduced under a Creative Commons License |
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