Lefoulon, C., Waghmare, S. , Karnik, R. and Blatt, M. R. (2018) Gating control and K+ uptake by the KAT1 K+ channel leaveraged through membrane anchoring of the trafficking protein SYP121. Plant, Cell and Environment, 41(11), pp. 2668-2677. (doi: 10.1111/pce.13392) (PMID:29940699) (PMCID:PMC6220998)
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Abstract
Vesicle traffic is tightly coordinated with ion transport for plant cell expansion through physical interactions between subsets of vesicle‐trafficking (so‐called SNARE) proteins and plasma membrane Kv channels, including the archetypal inward‐rectifying K+ channel, KAT1 of Arabidopsis. Ion channels open and close rapidly over milliseconds, whereas vesicle fusion events require many seconds. Binding has been mapped to conserved motifs of both the Kv channels and the SNAREs, but knowledge of the temporal kinetics of their interactions, especially as it might relate to channel gating and its coordination with vesicle fusion remains unclear. Here we report that the SNARE SYP121 promotes KAT1 gating through a persistent interaction that alters the stability of the channel, both in its open and closed states. We show, too, that SYP121 action on the channel open state requires SNARE anchoring in the plasma membrane. Our findings indicate that SNARE binding confers a conformational bias that encompasses the microscopic kinetics of channel gating, with leverage applied through the SNARE anchor in favor of the open channel.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Blatt, Professor Michael and Karnik, Dr Rucha and Lefoulon, Dr Cecile and Waghmare, Dr Sakharam |
| Creator Roles: | Lefoulon, C.Formal analysis, Writing – original draft, Writing – review and editing Blatt, M. R.Formal analysis, Writing – original draft, Writing – review and editing |
| Authors: | Lefoulon, C., Waghmare, S., Karnik, R., and Blatt, M. R. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Plant, Cell and Environment |
| Publisher: | Wiley |
| ISSN: | 0140-7791 |
| ISSN (Online): | 1365-3040 |
| Published Online: | 25 June 2018 |
| Copyright Holders: | Copyright © 2018 The Authors |
| First Published: | First published in Plant, Cell and Environment 41(11): 2668-2677 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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