Detection of circulating tumor cell clusters in human glioblastoma

Krol, I. et al. (2018) Detection of circulating tumor cell clusters in human glioblastoma. British Journal of Cancer, 119, pp. 487-491. (doi: 10.1038/s41416-018-0186-7) (PMID:30065256) (PMCID:PMC6134152)

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Human glioblastoma (GBM) is a highly aggressive, invasive and hypervascularised malignant brain cancer. Individual circulating tumour cells (CTCs) are sporadically found in GBM patients, yet it is unclear whether multicellular CTC clusters are generated in this disease and whether they can bypass the physical hurdle of the blood–brain barrier. Here, we assessed CTC presence and composition at multiple time points in 13 patients with progressing GBM during an open-label phase 1/2a study with the microtubule inhibitor BAL101553. We observe CTC clusters ranging from 2 to 23 cells and present at multiple sampling time points in a GBM patient with pleomorphism and extensive necrosis, throughout disease progression. Exome sequencing of GBM CTC clusters highlights variants in 58 cancer-associated genes including ATM, PMS2, POLE, APC, XPO1, TFRC, JAK2, ERBB4 and ALK. Together, our findings represent the first evidence of the presence of CTC clusters in GBM.

Item Type:Articles
Additional Information:Research in the Aceto lab is supported by the European Research Council, the Swiss National Science Foundation, the Swiss Cancer League, the Basel Cancer League, Basilea Pharmaceutica International Ltd., the two Cantons of Basel through the ETH Zürich, and the University of Basel.
Glasgow Author(s) Enlighten ID:Evans, Professor Jeff
Authors: Krol, I., Castro-Giner, F., Maurer, M., Gkountela, S., Szczerba, B., Scherrer, R., Coleman, N., Carreira, S., Bachmann, F., Anderson, S., Engelhardt, M., Lane, H., Evans, T. R. J., Plummer, R., Kristeleit, R., Lopez, J., and Aceto, N.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:British Journal of Cancer
Publisher:Nature Publishing Group
ISSN (Online):1532-1827
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in British Journal of Cancer 119:487-491
Publisher Policy:Reproduced under a Creative Commons License

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