Keating, C. , Hughes, D., Mahony, T., Cysneiros, D., Ijaz, U.Z. , Smith, C.J. and O‘Flaherty, V. (2018) Cold adaptation and replicable microbial community development during long-term low temperature anaerobic digestion treatment of synthetic sewage. FEMS Microbiology Ecology, 94(7), fiy095. (doi: 10.1093/femsec/fiy095) (PMID:29846574) (PMCID:PMC5995215)
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Abstract
The development and, activity of a cold-adapting microbial community was monitored during low temperature anaerobic digestion (LtAD) treatment of wastewater. Two replicate hybrid anaerobic sludge bed-fixed-film reactors treated a synthetic sewage wastewater at 12°C, at organic loading rates of 0.25–1.0 kg Chemical Oxygen Demand (COD) m−3 d−1, over 889 days. The inoculum was obtained from a full-scale AD reactor, which was operated at 37˚C. Both LtAD reactors readily degraded the influent with COD removal efficiencies regularly exceeding 78% for both the total and soluble COD fractions. The biomass from both reactors was sampled temporally and tested for activity against hydrolytic and methanogenic substrates at 12˚C and 37˚C. Data indicated that significantly enhanced low-temperature hydrolytic and methanogenic activity developed in both systems. For example, the hydrolysis rate constant (K) at 12°C had increased 20–30-fold by comparison to the inoculum by day 500. Substrate affinity also increased for hydrolytic substrates at low temperature. Next generation sequencing demonstrated that a shift in community structure occurred over the trial, involving a 1-log-fold change in 25 SEQS (OTU-free approach) from the inoculum. Microbial community structure changes and process performance were replicable in the LtAD reactors.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Keating, Dr Ciara and Smith, Professor Cindy and Ijaz, Dr Umer |
Authors: | Keating, C., Hughes, D., Mahony, T., Cysneiros, D., Ijaz, U.Z., Smith, C.J., and O‘Flaherty, V. |
College/School: | College of Science and Engineering > School of Engineering > Infrastructure and Environment |
Journal Name: | FEMS Microbiology Ecology |
Publisher: | Oxford University Press |
ISSN: | 0168-6496 |
ISSN (Online): | 1574-6941 |
Published Online: | 25 May 2018 |
Copyright Holders: | Copyright © 2018 FEMS |
First Published: | First published in FEMS Microbiology Ecology 94(7): fiy095 |
Publisher Policy: | Reproduced under a Creative Commons License |
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