Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health

Borsanelli, A. C., Lappin, D. F., Viora, L. , King, G. , Bennett, D., Dutra, I. S. and Riggio, M. P. (2018) Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health. Research in Veterinary Science, 118, pp. 439-443. (doi: 10.1016/j.rvsc.2018.04.008) (PMID:29729606)

Abstract

Bovine periodontitis is a progressive and purulent infection associated with an anaerobic subgingival biofilm, which induces irreversible damage to the dentition of affected animals. The aetiopathogenesis of the disease is unclear and treatment and control of the disease process in cattle are almost unknown. The aim of this study was to investigate the innate immune response by quantifying expression of Toll-like receptor (TLR) and cytokine genes in gingival tissue samples from cattle with and without periodontitis. Postmortem biopsies of gingival tissues were collected from 20 cattle with periodontitis and 20 cattle with no clinical signs of periodontal lesions. Tissue expression of TLR2, TLR4, TNF-α, IFN-γ, IL-1β and IL-4 genes were determined using quantitative real-time PCR. Statistically significant increases in mRNA levels encoding TLR2 (p = 0.025), TLR4 (p = 0.037), TNF-α (p = 0.025), IFN-γ (p = 0.014), IL-1β (p < 0.001) and IL-4 (p = 0.014) were observed in animals with periodontitis when compared to periodontally healthy animals. Increased levels of TLRs and inflammatory cytokines in periodontal tissue indicate an induction of the innate immune response of cattle and suggest that a substantial microbial challenge may be involved in the aetiopathogenesis of bovine periodontitis.

• Item Type: Articles
• Additional Information: This work was supported by the Sao Paulo Research Foundation (FAPESP) through the award of a doctoral scholarship (Process FAPESP2015/06917-9).
• Keywords: Bovine, cytokines, innate immune response, periodontitis, toll-like receptors.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Lappin, Dr David and Riggio, Professor Marcello and Viora, Dr Lorenzo and Bennett, Professor David and King, Mr George
• Authors: Borsanelli, A. C., Lappin, D. F., Viora, L., King, G., Bennett, D., Dutra, I. S., and Riggio, M. P.
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing; College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Research in Veterinary Science
• Publisher: Elsevier
• ISSN: 0034-5288
• ISSN (Online): 1532-2661
• Published Online: 25 April 2018
• Copyright Holders: Copyright © 2018 Elsevier Ltd.
• First Published: First published in Research in Veterinary Science 118:439-443
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher