Ranford-Cartwright, L. and Walliker, D. (1999) Intragenic recombinants of Plasmodium falciparum identified by in situ polymerase chain reaction. Molecular and Biochemical Parasitology, 102(1), pp. 13-20. (doi: 10.1016/S0166-6851(99)00069-9) (PMID:10477172)
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Abstract
We report an in situ PCR technique for visualising amplified DNA of blood forms of Plasmodium falciparum on microscope slides by fluorescence microscopy. The method is used to assess the changes in frequency of different alleles of the MSP1 gene in cultures of the progeny of a cross. We show that parasites with a recombinant form of this protein possess an initial growth advantage before declining in numbers over the long-term.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Ranford-Cartwright, Dr Lisa |
| Authors: | Ranford-Cartwright, L., and Walliker, D. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Molecular and Biochemical Parasitology |
| Publisher: | Elsevier |
| ISSN: | 0166-6851 |
| ISSN (Online): | 1872-9428 |
| Published Online: | 03 August 1999 |
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