Immunoassay multiplexing on a complementary metal oxide semiconductor photodiode array

Nagy, B., Al-Rawhani, M. A., Cheah, B. C., Barrett, M. P. and Cumming, D. R.S. (2018) Immunoassay multiplexing on a complementary metal oxide semiconductor photodiode array. ACS Sensors, 3(5), pp. 953-959. (doi: 10.1021/acssensors.7b00972) (PMID:29652490) (PMCID:PMC5974700)

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Scalable immunoassay multiplexing offers a route to creating rapid point-of-care (POC) diagnostics. We present a method for multiplexing immunoassays on the surface of a complementary metal oxide semiconductor (CMOS) sensor array integrated circuit (IC) without the use of physical separators such as wells or channels. Major advantages of using a CMOS sensor array include low mass-manufacturing costs, the possibility to multiplex multiple assays on a single IC, and improved signal when averaging multiple sensors, along with providing a platform where wash steps can be incorporated to maximize selectivity and sensitivity compared to paper based lateral flow immunoassay. The device was able to differentiate between samples containing either, neither, or both rabbit anti-mouse (RAM) antibodies and/or anti-HIV gp120 antibodies in serum using a gold-nanoparticle promoted silver enhancement immunoassay. HIV antibody concentrations down to 100 μg/mL were readily detected, which is three times lower than those typically found in infected humans (300–500 μg/mL), and the limit of detection was 10 μg/mL.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Al-Rawhani, Dr Mohammed and Cumming, Professor David and Cheah, Boon Chong and NAGY, BENCE and Barrett, Professor Michael
Authors: Nagy, B., Al-Rawhani, M. A., Cheah, B. C., Barrett, M. P., and Cumming, D. R.S.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
Journal Name:ACS Sensors
Publisher:American Chemical Society
ISSN (Online):2379-3694
Published Online:13 April 2018
Copyright Holders:Copyright © 2018 American Chemical Society
First Published:First published in ACS Sensors 3(5):953-959
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.5525/gla.researchdata.602

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
605821The Multi-Corder: Poly-Sensor TechnologyDavid CummingEngineering and Physical Sciences Research Council (EPSRC)EP/K021966/1ENG - ENGINEERING ELECTRONICS & NANO ENG
371799The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOTR)104111/Z/14/Z & AIII - PARASITOLOGY