Nagy, B., Al-Rawhani, M. A., Cheah, B. C., Barrett, M. P. and Cumming, D. R.S. (2018) Immunoassay multiplexing on a complementary metal oxide semiconductor photodiode array. ACS Sensors, 3(5), pp. 953-959. (doi: 10.1021/acssensors.7b00972) (PMID:29652490) (PMCID:PMC5974700)
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Abstract
Scalable immunoassay multiplexing offers a route to creating rapid point-of-care (POC) diagnostics. We present a method for multiplexing immunoassays on the surface of a complementary metal oxide semiconductor (CMOS) sensor array integrated circuit (IC) without the use of physical separators such as wells or channels. Major advantages of using a CMOS sensor array include low mass-manufacturing costs, the possibility to multiplex multiple assays on a single IC, and improved signal when averaging multiple sensors, along with providing a platform where wash steps can be incorporated to maximize selectivity and sensitivity compared to paper based lateral flow immunoassay. The device was able to differentiate between samples containing either, neither, or both rabbit anti-mouse (RAM) antibodies and/or anti-HIV gp120 antibodies in serum using a gold-nanoparticle promoted silver enhancement immunoassay. HIV antibody concentrations down to 100 μg/mL were readily detected, which is three times lower than those typically found in infected humans (300–500 μg/mL), and the limit of detection was 10 μg/mL.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Al-Rawhani, Dr Mohammed and Cumming, Professor David and Cheah, Boon Chong and NAGY, BENCE and Barrett, Professor Michael |
| Authors: | Nagy, B., Al-Rawhani, M. A., Cheah, B. C., Barrett, M. P., and Cumming, D. R.S. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering |
| Journal Name: | ACS Sensors |
| Publisher: | American Chemical Society |
| ISSN: | 2379-3694 |
| ISSN (Online): | 2379-3694 |
| Published Online: | 13 April 2018 |
| Copyright Holders: | Copyright © 2018 American Chemical Society |
| First Published: | First published in ACS Sensors 3(5):953-959 |
| Publisher Policy: | Reproduced under a Creative Commons License |
| Data DOI: | 10.5525/gla.researchdata.602 |
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