Maternal dietary supplementation with saturated, but not monounsaturated or polyunsaturated fatty acids, leads to tissue-specific inhibition of offspring Na+,K+-ATPase

Armitage, J. A., Gupta, S., Wood, C., Jensen, R. I., Samuelsson, A.-M., Fuller, W. , Shattock, M. J., Poston, L. and Taylor, P. D. (2008) Maternal dietary supplementation with saturated, but not monounsaturated or polyunsaturated fatty acids, leads to tissue-specific inhibition of offspring Na+,K+-ATPase. Journal of Physiology, 586(20), pp. 5013-5022. (doi: 10.1113/jphysiol.2008.157818) (PMID:18718984)

Abstract

In rats, a maternal diet rich in lard is associated with reduced Na+,K+‐ATPase activity in adult offspring kidney. We have addressed the role of different fatty acids by evaluating Na+,K+‐ATPase activity in offspring of dams fed diets rich in saturated (SFA), monounsaturated (MUFA) or polyunsaturated (PUFA) fatty acids. Female Sprague–Dawley rats were fed, during pregnancy and suckling, a control diet (4% w/w corn oil) or a fatty acid supplemented diet (24% w/w). Offspring were reared on chow (4% PUFA) and studied at 6 months. mRNA expression (real‐time PCR) of Na+,K+‐ATPase α subunit and protein expression of Na+,K+‐ATPase subunits (Western blot) were assessed in kidney and brain. Na+,K+‐ATPase activity was reduced in kidney (P < 0.05 versus all groups) and brain (P < 0.05 versus control and MUFA offspring) of the SFA group. Neither Na+,K+‐ATPase α1 subunit mRNA expression, nor protein expression of total α, α1, α2, α3 or β1 subunits were significantly altered in kidney in any dietary group. In brains of SFA offspring α1 mRNA expression (P < 0.05) was reduced compared with MUFA and PUFA offspring, but not controls. Also in brain, SFA offspring demonstrated reduced (P < 0.05) α1 subunit protein and increased phosphorylation (P < 0.05) of the Na+,K+‐ATPase modulating protein phospholemman at serine residue 63 (S63 PLM). Na+,K+‐ATPase activity was similar to controls in heart and liver. In utero and neonatal exposure to a maternal diet rich in saturated fatty acids is associated with altered activity and expression of Na+,K+‐ATPase in adulthood, but mechanisms appear tissue specific.

• Item Type: Articles
• Additional Information: JAA is a National Heart Foundation and Monash Logan Fellow.This work is supported by the British Heart Foundation. L.P. issupported by Tommys the Baby Charity. A.-M.S. is supportedby EARNEST.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Fuller, Professor Will
• Authors: Armitage, J. A., Gupta, S., Wood, C., Jensen, R. I., Samuelsson, A.-M., Fuller, W., Shattock, M. J., Poston, L., and Taylor, P. D.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Journal of Physiology
• ISSN: 0022-3751
• ISSN (Online): 1469-7793
• Published Online: 21 August 2008