Comparison of urine and plasma peptidome indicates selectivity in renal peptide handling

Magalhães, P. et al. (2018) Comparison of urine and plasma peptidome indicates selectivity in renal peptide handling. Proteomics Clinical Applications, 12(5), 1700163. (doi: 10.1002/prca.201700163) (PMID:29611317)

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Purpose: Urine is considered to be produced predominantly as a result of plasma filtration in the kidney. However, the origin of the native peptides present in urine has never been investigated in detail. Therefore, we aimed to obtain a first insight into the origin of urinary peptides based on a side‐by‐side comprehensive analysis of the plasma and urine peptidome. Methods: Twenty‐two matched urine and plasma samples were analyzed for their peptidome using capillary electrophoresis coupled to mass spectrometry (CE‐MS; for relative quantification) and CE‐ or LC coupled to tandem mass spectrometry (CE‐ or LC‐ MS/MS; for peptide identification). The overlap and association of abundance of the different peptides present in these two body fluids were evaluated. Results: We were able to identify 561 plasma and 1461 urinary endogenous peptides. Only 90 peptides were detectable in both urine and plasma. No significant correlation was found when comparing the abundance of these common peptides, with the exception of collagen fragments. This observation was also supported when comparing published peptidome data from both plasma and urine. Conclusions and clinical relevance: Most of the plasma peptides are not detectable in urine, possibly due to tubular reabsorption. The majority of urinary peptides may in fact originate in the kidney. The notable exception is collagen fragments, which indicates potential selective exclusion of these peptides from tubular reabsorption. Experimental verification of this hypothesis is warranted.

Item Type:Articles
Keywords:Clinical biochemistry.
Glasgow Author(s) Enlighten ID:Mischak, Professor Harald and Carrick, Dr Emma
Authors: Magalhães, P., Pontillo, C., Pejchinovski, M., Siwy, J., Krochmal, M., Makridakis, M., Carrick, E., Klein, J., Mullen, W., Jankowski, J., Vlahou, A., Mischak, H., Schanstra, J. P., Zürbig, P., and Pape, L.
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
Journal Name:Proteomics Clinical Applications
ISSN (Online):1862-8354
Published Online:03 April 2018
Copyright Holders:Copyright © 2018 Wiley-VCH Verlag GmbH and Co. KGaA
First Published:First published in Proteomics Clinical Applications 12(5): 1700163
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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