Casson, J., O'Kane, S., Smith, C.-A. , Dalby, M. J. and Berry, C. C. (2018) Interleukin 6 plays a role in the migration of magnetically levitated mesenchymal stem cells spheroids. Applied Sciences, 8(3), 412. (doi: 10.3390/app8030412)
|
Text
159093.pdf - Published Version Available under License Creative Commons Attribution. 2MB |
Abstract
Mesenchymal stem cells (MSCs) reside quiescently within a specialised ‘niche’ environment in the bone marrow. However, following appropriate signalling cues, MSCs mobilise and migrate out from the niche, typically toward either sites of injury (a regenerative response) or toward primary tumours (an intrinsic homing response, which promotes MSCs as cellular vectors for therapeutic delivery). To date, very little is known about MSC mobilisation. By adopting a 3D MSC niche model, whereby MSC spheroids are cultured within a type I collagen gel, recent studies have highlighted interleukin-6 (IL-6) as a key cytokine involved in MSC migration. Herein, the ability of IL-6 to induce MSC migration was further investigated, and the key matrix metalloproteinases used to effect cell mobilisation were identified. Briefly, the impact of IL-6 on the MSC migration in a two-dimensional model systems was characterised—both visually using an Ibidi chemotaxis plate array (assessing for directional migration) and then via a standard 2D monolayer experiment, where cultured cells were challenged with IL-6 and extracted media tested using an Abcam Human MMP membrane antibody array. The 2D assay displayed a strong migratory response toward IL-6 and analysis of the membrane arrays data showed significant increases of several key MMPs. Both data sets indicated that IL-6 is important in MSC mobilisation and migration. We also investigated the impact of IL-6 induction on MSCs in 3D spheroid culture, serving as a simplistic model of the bone marrow niche, characterised by fluorescently tagged magnetic nanoparticles and identical membrane antibody arrays. An increase in MMP levels secreted by cells treated with 1 ng/mL IL-6 versus control conditions was noted in addition to migration of cells away from the central spheroid mass.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Berry, Dr Catherine and Dalby, Professor Matthew and Casson, Jake and Smith, Mrs Carol-Anne |
Authors: | Casson, J., O'Kane, S., Smith, C.-A., Dalby, M. J., and Berry, C. C. |
College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Applied Sciences |
Publisher: | MDPI |
ISSN: | 2076-3417 |
ISSN (Online): | 2076-3417 |
Published Online: | 11 March 2018 |
Copyright Holders: | Copyright © 2018 The Authors |
First Published: | First published in Applied Sciences 8(3):412 |
Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record