James, A. B. , Sullivan, S. and Nimmo, H. G. (2018) Global spatial analysis of Arabidopsis natural variants implicates 5’UTR splicing of LATE ELONGATED HYPOCOTYL in responses to temperature. Plant, Cell and Environment, 41(7), pp. 1524-1538. (doi: 10.1111/pce.13188) (PMID:29520807) (PMCID:PMC6033021)
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Abstract
How plants perceive and respond to temperature remains an important question in the plant sciences. Temperature perception and signal transduction may occur through temperature‐sensitive intra‐molecular folding of primary mRNA transcripts. Recent studies suggested a role for retention of the first intron in the 5’UTR of the clock component LATE ELONGATED HYPOCOTYL (LHY) in response to changes in temperature. Here we identified a set of haplotypes in the LHY 5’UTR, examined their global spatial distribution and obtained evidence that haplotype can affect temperature‐dependent splicing of LHY transcripts. Correlations of haplotype spatial distributions with global bioclimatic variables and altitude point to associations with annual mean temperature and temperature fluctuation. Relatively rare relict type accessions correlate with lower mean temperature and greater temperature fluctuation and the spatial distribution of other haplotypes may be informative of evolutionary processes driving colonisation of ecosystems. We propose that haplotypes may possess distinct 5’UTR pre‐mRNA folding thermodynamics and/or specific biological stabilities based around the binding of trans‐acting RNA splicing factors, a consequence of which is scalable splicing sensitivity of a central clock component that is likely tuned to specific temperature environments.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Nimmo, Professor Hugh and James, Dr Allan and Sullivan, Dr Stuart |
Authors: | James, A. B., Sullivan, S., and Nimmo, H. G. |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Plant, Cell and Environment |
Publisher: | Wiley |
ISSN: | 0140-7791 |
ISSN (Online): | 1365-3040 |
Published Online: | 08 March 2018 |
Copyright Holders: | Copyright © 2018 John Wiley and Sons Ltd |
First Published: | First published in Plant, Cell and Environment 41(7):1524-1538 |
Publisher Policy: | Reproduced under a Creative Commons License |
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