Xue, G.P., Patel, M., Johnson, J.S., Smyth, D.J. and Vickers, C.E. (2003) Selectable marker-free transgenic barley producing a high level of cellulase (1,4-β-glucanase) in developing grains. Plant Cell Reports, 21(11), pp. 1088-1094. (doi: 10.1007/s00299-003-0627-4) (PMID:12836003)
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Abstract
The use of barley grains as bioreactors for high-level production of cellulase (1,4-β-glucanase) was investigated. A hybrid cellulase gene, cel-hyb1, driven by the rice GluB-1 promoter was expressed specifically in developing endosperm. Codon usage optimisation of cel-hyb1 increased its expression in barley grains 527-fold and led to cellulase production of up to 1.5% of total grain protein. CEL-HYB1 enzyme in barley grains was highly stable during post-harvest storage. Selectable marker gene (hph) was subsequently eliminated from transgenic lines through segregation of hph from synthetic cel-hyb1 (syn.cel-hyb1) in T1 progeny, using a binary plasmid containing hph and syn.cel-hyb1 in separate T-DNAs. These data suggest that barley grains can potentially be used for the commercial production of cellulase.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Smyth, Dr Danielle |
| Authors: | Xue, G.P., Patel, M., Johnson, J.S., Smyth, D.J., and Vickers, C.E. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Plant Cell Reports |
| Publisher: | Springer |
| ISSN: | 0721-7714 |
| ISSN (Online): | 1432-203X |
| Published Online: | 16 April 2003 |
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