Cultivation of Heligmosomoides polygyrus: an immunomodulatory nematode parasite and its secreted products

Johnston, C. J.C., Robertson, E., Harcus, Y., Grainger, J. R., Coakley, G., Smyth, D. J. , McSorley, H. J. and Maizels, R. (2015) Cultivation of Heligmosomoides polygyrus: an immunomodulatory nematode parasite and its secreted products. Journal of Visualized Experiments(98), e52412. (doi: 10.3791/52412) (PMID:25867600) (PMCID:PMC4401400)

[img]
Preview
Text
158623.pdf - Published Version
Available under License Creative Commons Attribution.

380kB

Abstract

Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).

Item Type:Articles
Additional Information:The authors are funded by the Wellcome Trust through the Edinburgh Clinical Academic Track (C.J.C.J.) and Programme Grant (Y.H., and R.M.M.) and Fellowship (J.R.G) funding, the BBSRC (G.C.), the American Asthma Foundation (E.R., H.J.McS. and R.M.M.), Asthma UK (H.J.McS.) and the Rainin Foundation (D.J.S. and R.M.M.).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Smyth, Dr Danielle and Coakley, Miss Gillian and Maizels, Professor Rick
Authors: Johnston, C. J.C., Robertson, E., Harcus, Y., Grainger, J. R., Coakley, G., Smyth, D. J., McSorley, H. J., and Maizels, R.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Journal of Visualized Experiments
Publisher:JoVE
ISSN:1940-087X
ISSN (Online):1940-087X
Copyright Holders:Copyright © 2015
First Published:First published in Journal of Visualized Experiments 98:e52412
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record