Investigating the role of AMPK in inflammation

Mancini, S. J. and Salt, I. P. (2018) Investigating the role of AMPK in inflammation. In: Neumann, D. and Viollet, B. (eds.) AMPK: Methods and Protocols. Series: Methods in Molecular Biology, 1732. Humana Press, pp. 307-319. ISBN 9781493975976 (doi: 10.1007/978-1-4939-7598-3_20)

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In addition to the well-characterized role of AMPK in the regulation of nutrient metabolism, it is increasingly clear that AMPK activation has multiple actions on inflammatory signalling. Here we describe methods to identify effects of AMPK activity on pro-inflammatory signalling, specifically (1) the nuclear localization of the key inflammatory mediators nuclear factor-κB (NFκB) and phosphorylated c-Jun N-terminal kinase (JNK), (2) preparation of conditioned medium to analyze the secretion of cytokines/chemokines, and (3) the pro-inflammatory adhesion of leukocytes to cultured cells.

Item Type:Book Sections
Glasgow Author(s) Enlighten ID:Mancini, Dr Sarah and Salt, Dr Ian
Authors: Mancini, S. J., and Salt, I. P.
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Publisher:Humana Press

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