Creating tissue on chip constructs in microtitre plates for drug discovery

Reboud, J. , Macdonald, N.P., Menachery, A. and Cooper, J.M. (2018) Creating tissue on chip constructs in microtitre plates for drug discovery. RSC Advances, 8, pp. 9603-9610. (doi: 10.1039/C8RA00849C)

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We report upon a novel coplanar dielectrophoresis (DEP) based cell patterning system for generating transferrable hepatic cell constructs, resembling a liver-lobule, in culture. The use of paper reinforced gel substrates provided sufficient strength to enable these constructs to be transfered into 96-well plates for long term functional studies, including in the future, drug development studies. Experimental results showed that hepatic cells formed DEP field-induced structures corresponding to an array of lobule-mimetic patterns. Hepatic viability was observed over a period of 3 days by the use of a fluorescent cell staining technique, whilst the liver specific functionality of albumin secretion showed a significant enhancement due to the layer patterning of cell lines (HepG2/C3A), compared to 2D patterned cells and un-patterned control. This “build and transfer” concept could, in future, also be adapted for the layer-by-layer construction of organs-on-chip in microtitre formats.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan and Reboud, Dr Julien
Authors: Reboud, J., Macdonald, N.P., Menachery, A., and Cooper, J.M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:RSC Advances
Publisher:Royal Society of Chemistry
ISSN (Online):2046-2069
Published Online:06 March 2018
Copyright Holders:Copyright © 2018 The Royal Society of Chemistry
First Published:First published in RSC Advances 8:9603-9610
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
Industrial CASE Account - Glasgow 2008EP/G501408/1