Busin, V. , Burgess, S. and Shu, W. (2016) A Novel Multi-pad Paper Plate (MP3) Based Assays for Rapid Animal Disease Diagnostics. In: 30th Eurosensors Conference, EUROSENSORS 2016, Budapest, Hungary, 4-7 Sept 2016, pp. 1418-1421.
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Abstract
This study describes a novel fabrication method of a multi-pad paper plate (MP3) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwich ELISA assay for detection of the acute phase protein bovine haptoglobin, allowing assay optimisation in the new format. The optimised protocol was also applied to detection of haptoglobin in bovine serum, demonstrating significant correlation with the traditional lab-based ELISA.
| Item Type: | Conference Proceedings |
|---|---|
| Additional Information: | Published in Procedia Engineering, 168:1418-1421 |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Busin, Valentina |
| Authors: | Busin, V., Burgess, S., and Shu, W. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Procedia Engineering |
| Publisher: | Elsevier |
| ISSN: | 1877-7058 |
| ISSN (Online): | 1877-7058 |
| Published Online: | 04 January 2017 |
| Copyright Holders: | Copyright © 2016 The Authors |
| First Published: | First published in Procedia Engineering 168:1418-1421 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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