Yuan, X. et al. (2018) Effect of laser irradiation on cell function and its implications in Raman spectroscopy. Applied and Environmental Microbiology, 84(8), e02508-17. (doi: 10.1128/AEM.02508-17) (PMID:29427427) (PMCID:PMC5881070)
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Abstract
Lasers are instrumental in advanced bioimaging and Raman spectroscopy. However, they are also well known for their destructive effects on living organisms, leading to concerns about the adverse effects of laser technologies. To implement Raman spectroscopy for cell analysis and manipulation, such as Raman activated cell sorting, it is crucial to identify non-destructive conditions for living cells. Here, we evaluated quantitatively the effect of 532 nm laser irradiation on bacterial cell fate and growth at the single-cell level. Using a purpose-built microfluidic platform, we were able to quantify the growth characteristics i.e. specific growth rate and lag time of individual cells as well as the survival rate of a population in conjunction with Raman spectroscopy. Representative Gram-negative and Gram-positive species show a similar trend in response to laser irradiation dose. Laser irradiation could compromise physiological function of cells and the degree of destruction is both dose and strain dependent, ranging from reduced cell growth to a complete loss of cell metabolic activity and finally to physical disintegration. Gram-positive bacterial cells are more susceptible than Gram-negative bacterial strains to irradiation-induced damage. By directly correlating Raman acquisition with single cell growth characteristics, we provide evidence of non-destructive characteristics of Raman spectroscopy on individual bacterial cells. However, while strong Raman signals can be obtained without causing cell death, the variety of responses from different strains and from individual cells justify careful evaluation of Raman acquisition conditions if cell viability is critical.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Yin, Professor Huabing and Song, Dr Yanqing and Yuan, Dr Xiaofei and Glidle, Dr Andrew and Cooper, Professor Jonathan and Ijaz, Dr Umer |
| Authors: | Yuan, X., Song, Y., Song, Y., Xu, J., Wu, Y., Glidle, A., Cusack, M., Ijaz, U. Z., Cooper, J. M., Huang, W. E., and Yin, H. |
| College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering College of Science and Engineering > School of Engineering > Infrastructure and Environment |
| Journal Name: | Applied and Environmental Microbiology |
| Publisher: | American Society for Microbiology. |
| ISSN: | 0099-2240 |
| ISSN (Online): | 1098-5336 |
| Published Online: | 02 February 2018 |
| Copyright Holders: | Copyright © 2018 Yuan et al. |
| First Published: | First published in Applied and Environmental Microbiology 84(8):e02508-17 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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