Johnstone, I.L., Hughes, S.G. and Clutterbuck, A.J. (1985) Cloning an Aspergillus nidulans developmental gene by transformation. EMBO Journal, 4(5), pp. 1307-1311. (PMID:3891328) (PMCID:PMC554341)
Full text not currently available from Enlighten.
Abstract
We have developed a transformation system for Aspergillus nidulans giving a frequency of transformation high enough to screen a gene bank from which we were able to isolate and clone the A. nidulans developmental gene brlA by visual selection. The vector contains the selective marker argB+, and with it a frequency of transformation of 500 stable transformants/micrograms plasmid DNA can regularly be achieved. The evidence suggests that transformation is by integration but spontaneous excision of integrated plasmids is apparently frequent enough to allow the recovery of transforming plasmids in Escherichia coli.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Johnstone, Professor Iain and Clutterbuck, Dr John |
Authors: | Johnstone, I.L., Hughes, S.G., and Clutterbuck, A.J. |
Subjects: | Q Science > QH Natural history > QH426 Genetics |
College/School: | College of Medical Veterinary and Life Sciences > School of Life Sciences College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | EMBO Journal |
Publisher: | IRL Press |
ISSN: | 0261-4189 |
ISSN (Online): | 1460-2075 |
University Staff: Request a correction | Enlighten Editors: Update this record