Lu, J., Liu, Q., Zhao, X., Gao, R., Cong, W. , Wang, Y. and Luo, G. (2010) Simultaneous RP-LC analysis of the prodrug Scu-PEG and its metabolite scutellarin in rat plasma. Chromatographia, 71(5-6), pp. 511-515. (doi: 10.1365/s10337-009-1468-2)
Full text not currently available from Enlighten.
Abstract
Scutellarin is released from the prodrug Scu-PEG after i.v. administration to rats. To investigate changes in the amount of the prodrug in vivo, a liquid chromatographic method for simultaneous analysis of Scu-PEG and scutellarin in rat plasma was developed and validated. A C18 column was used with a binary mobile phase gradient prepared from acetonitrile and 0.3% aqueous formic acid solution. The detection wavelength was 334 nm and the column temperature 40 °C. Linearity was excellent, with R 2 no less than 0.999. Intra-assay and inter-assay precision, as RSD, were less than 6.4 and 4.7%, respectively, for scutellarin and less than 2.8 and 2.9% for Scu-PEG. The method is applicable to pharmacokinetic study the prodrug and its metabolite in rats after i.v. administration.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cong, Dr Wenjuan |
| Authors: | Lu, J., Liu, Q., Zhao, X., Gao, R., Cong, W., Wang, Y., and Luo, G. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
| Journal Name: | Chromatographia |
| Publisher: | Springer |
| ISSN: | 0009-5893 |
| ISSN (Online): | 1612-1112 |
| Published Online: | 02 February 2010 |
University Staff: Request a correction | Enlighten Editors: Update this record
Altmetric
Altmetric