Yang, Z. , Xu, G., Reboud, J. , Ali, S. A., Kaur, G., McGiven, J., Boby, N., Gupta, P. K., Chaudhuri, P. and Cooper, J. M. (2018) Rapid veterinary diagnosis of bovine reproductive infectious diseases from semen using paper-origami DNA microfluidics. ACS Sensors, 3(2), pp. 403-409. (doi: 10.1021/acssensors.7b00825) (PMID:29322764)
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Abstract
The health and well-being of cattle is a significant concern for global agricultural output. In dairy production within low and middle income countries (LMICs), there is a significant biosensing challenge in detecting sexually transmitted infection (STI) pathogens during animal husbandry, due in part to difficulties associated with the limited infrastructure for veterinary medicine. Here we demonstrate low-cost, multiplexed and sample-to-answer paper-origami tests for the detection of three bovine infectious reproductive diseases in semen samples, collected at a test site in rural India. Pathogen DNA from one viral pathogen, Bovine Herpes virus-1 (BoHV-1) and two bacteria (Brucella and Leptospira) was extracted, amplified (using loop-mediated isothermal amplification, LAMP) and detected fluorescently, enabling <1 pg (~ from 115 to 274 copies per reaction) of target genomic DNA to be measured. Data was collected as a fluorescence signal either visually, using a low-cost hand-held torch, or digitally with a mobile-phone camera. Limits of detection and sensitivities of the paper-origami device for the three pathogens were also evaluated using pathogen-inoculated semen samples and were as few as 50 Leptospira organisms, 50 CFU Brucella and 1 TCID50. BoHV-1. Semen samples from elite bulls at a germplasm centre were also tested in double-blind tests, as a demonstrator for a low cost, user-friendly point-of-care sensing platform, for in-the-field resource-limited regions. The sensors showed excellent levels of sensitivity and specificity, and for the first of time a demonstrated ability of the application of paper-origami devices for the diagnosis multiple infectious diseases from semen samples.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cooper, Professor Jonathan and Reboud, Dr Julien and Xu, Mr Gaolian and Yang, Dr Zhugen |
| Authors: | Yang, Z., Xu, G., Reboud, J., Ali, S. A., Kaur, G., McGiven, J., Boby, N., Gupta, P. K., Chaudhuri, P., and Cooper, J. M. |
| College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | ACS Sensors |
| Publisher: | American Chemical Society |
| ISSN: | 2379-3694 |
| ISSN (Online): | 2379-3694 |
| Published Online: | 11 January 2018 |
| Copyright Holders: | Copyright © 2018 American Chemical Society |
| First Published: | First published in ACS Sensors 3(2): 403-409 |
| Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
| Data DOI: | 10.5525/gla.researchdata.568 |
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