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Thomas H. Ant, Christie S. Herd, Vincent Geoghegan, Ary A. Hoffmann, Steven P. Sinkins
The Wolbachia strain wAu provides highly efficient virus transmission blocking in Aedes aegypti
www.plospathogens.org
www.plospathogens.org
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/F8 1 Tf
1.0318 0 Td
[()30()]TJ
/F7 1 Tf
(.)Tj
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1.491 0 Td
[()6()9()-21()-13()12( )7(\n)]TJ
/F7 1 Tf
2.9877 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(26)Tj
0 g
(]:)Tj
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(rearing)Tj
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[()92()10(\r)14()4()6()13()18(\n)4()]TJ
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4.4617 0 Td
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3.3279 0 Td
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(ely)Tj
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1.0262 0 Td
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1.695 0 Td
(of)Tj
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[()]TJ
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(Mel)Tj
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(and)Tj
/F8 1 Tf
1.7348 0 Td
[()]TJ
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(26)Tj
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(factors,)Tj
3.1294 0 Td
(a)Tj
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(has)Tj
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[()]TJ
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4.0251 0 Td
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3.634 0 Td
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2.0012 0 Td
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(with)Tj
/F8 1 Tf
1.9956 0 Td
[()]TJ
/F7 1 Tf
(MelPop)Tj
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(density)Tj
3.101 0 Td
(in)Tj
/F8 1 Tf
1.0375 0 Td
[()-4()20()7()-3()11()10()]TJ
-33.766 -1.2983 Td
[(\n)4(\r)4()]TJ
1.2075 0 Td
[()6()15(\r)4()16()6()2()2()9( )11()2()]TJ
/F7 1 Tf
5.3858 0 Td
([)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
2.0749 0 Td
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4.0762 0 Td
(further)Tj
3.0331 0 Td
(studies)Tj
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2.4094 0 Td
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(a)Tj
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(role)Tj
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[()92()10(\r)14()4()6()13()18(\n)4()]TJ
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3.1011 0 Td
(regulation)Tj
4.3086 0 Td
([)Tj
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(but)Tj
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(see)Tj
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([)Tj
0.83 0.64 0.02 0 k
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(])Tj
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(and)Tj
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([)Tj
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(].)Tj
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(far,)Tj
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(only)Tj
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(a)Tj
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(repertoire)Tj
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(have)Tj
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(been)Tj
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(into)Tj
/F8 1 Tf
1.8368 0 Td
[()30()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.491 0 Td
[()6()9()-21()-13()12( )7(\n)]TJ
/F7 1 Tf
(.)Tj
3.2145 0 Td
(It)Tj
0.856 0 Td
(is)Tj
0.8504 0 Td
(important)Tj
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(to)Tj
1.0261 0 Td
(create)Tj
2.5909 0 Td
(and)Tj
1.7348 0 Td
(characterize)Tj
5.0286 0 Td
(further)Tj
3.0274 0 Td
(transinfections)Tj
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(in)Tj
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(this)Tj
1.6724 0 Td
(species)Tj
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(since)Tj
2.2394 0 Td
(they)Tj
1.9275 0 Td
(might)Tj
2.5965 0 Td
(provide)Tj
3.2882 0 Td
(improved)Tj
4.1102 0 Td
(characteristics)Tj
5.9017 0 Td
(such)Tj
2.0636 0 Td
(as)Tj
1.0148 0 Td
(viral)Tj
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(blocking)Tj
3.685 0 Td
(under)Tj
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4.1101 0 Td
(environmental)Tj
6.1115 0 Td
(conditions,)Tj
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(especially)Tj
4.0195 0 Td
(in)Tj
1.0318 0 Td
(hot)Tj
1.5534 0 Td
(climates)Tj
3.4923 0 Td
([)Tj
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(3)Tj
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(],)Tj
1.6044 0 Td
(and)Tj
1.7291 0 Td
(offer)Tj
2.0976 0 Td
(insights)Tj
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(into)Tj
1.8425 0 Td
(the)Tj
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(regulation)Tj
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(of)Tj
1.0148 0 Td
(intracellular)Tj
5.0569 0 Td
(density)Tj
3.1011 0 Td
(and)Tj
1.7291 0 Td
(its)Tj
1.1452 0 Td
(role)Tj
1.7688 0 Td
(in)Tj
1.0375 0 Td
(inducing)Tj
3.7928 0 Td
(pathogen)Tj
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(inhibition)Tj
4.2236 0 Td
(and)Tj
1.7291 0 Td
(effects)Tj
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(on)Tj
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(host)Tj
1.9105 0 Td
(fitness.)Tj
2.9933 0 Td
(Limitations)Tj
4.8302 0 Td
(are)Tj
1.4457 0 Td
(imposed)Tj
3.6453 0 Td
(by)Tj
1.1792 0 Td
(the)Tj
1.4684 0 Td
(technical)Tj
3.8154 0 Td
(demands)Tj
3.8608 0 Td
(of)Tj
1.0148 0 Td
(embryo)Tj
3.3051 0 Td
(cytoplasmic)Tj
4.972 0 Td
(transfer)Tj
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(by)Tj
1.1792 0 Td
(microinjection)Tj
6.1511 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4683 0 Td
(need)Tj
2.143 0 Td
(for)Tj
1.389 0 Td
(robust)Tj
2.7893 0 Td
(lab)Tj
1.4116 0 Td
(colonies)Tj
3.5036 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(insects)Tj
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(to)Tj
1.0261 0 Td
(be)Tj
1.1509 0 Td
(used)Tj
2.0579 0 Td
(as)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(source)Tj
2.8346 0 Td
(of)Tj
/F8 1 Tf
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[()92()10(\r)14()4()6()13()18(\n)4()]TJ
/F7 1 Tf
(.)Tj
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(While)Tj
/F8 1 Tf
2.6533 0 Td
[()]TJ
/F7 1 Tf
(AlbA)Tj
3.0047 0 Td
(and)Tj
/F8 1 Tf
1.7291 0 Td
[()]TJ
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(AlbB)Tj
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(are)Tj
1.4513 0 Td
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5.947 0 Td
[()30()]TJ
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1.4911 0 Td
[()11(\r)14()4()11()11(\n)4()-4( )7()-4()]TJ
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(,)Tj
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[()]TJ
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(AlbA)Tj
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[()]TJ
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(AlbB)Tj
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[()30()]TJ
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[()11(\r)14()4()11()11(\n)4()-4( )7()-4()]TJ
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1.8368 0 Td
[()30()]TJ
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(.)Tj
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[()6()9()-21()-13()12( )7(\n)]TJ
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(32)Tj
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[()]TJ
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[()-4()20()7()-3()11()10()18(\n)4(\r)4()]TJ
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[()10(\n)12()18()4(\r)4()16()]TJ
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[()-4()20()7()-3()11()10()18(\n)4(\r)4()]TJ
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[()6()9()-21()-13()12( )7(\n)]TJ
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[()]TJ
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[()]TJ
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(population-replacement)Tj
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(previously)Tj
4.3483 0 Td
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[()]TJ
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(AlbB)Tj
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(and)Tj
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1.7291 0 Td
[()]TJ
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(Mel)Tj
2.4435 0 Td
(transinfections.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 347.3007 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Results)Tj
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0 -1.4976 Td
(Strain)Tj
2.7685 0 Td
(generation)Tj
4.7055 0 Td
(and)Tj
1.7906 0 Td
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Q
q
1 j
1 J
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BT
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1 TL
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2.5965 0 Td
(embryonic)Tj
4.541 0 Td
(cytoplasmic)Tj
4.972 0 Td
(transfer)Tj
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[()92()10(\r)14()4()6()13()18(\n)4()]TJ
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4.456 0 Td
(transinfected)Tj
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(lines)Tj
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(carrying)Tj
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(strains)Tj
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/F7 1 Tf
(AlbA,)Tj
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[()]TJ
/F7 1 Tf
(AlbB,)Tj
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[()]TJ
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(Mel,)Tj
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[()]TJ
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(Au)Tj
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(same)Tj
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(host)Tj
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(background)Tj
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[()30()]TJ
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[()6()9()-21()-13()12( )7(\n)]TJ
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(the)Tj
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[()92()10(\r)14()4()6()13()18(\n)4()]TJ
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(strains)Tj
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(apart)Tj
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[()]TJ
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(Au)Tj
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(cytoplasmic)Tj
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(incompatibility)Tj
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(mosquitoes,)Tj
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[()]TJ
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