Baltierra Jasso, L., Morten, M. and Magennis, S. W. (2018) Sub-ensemble monitoring of DNA strand displacement using multiparameter single-molecule FRET. ChemPhysChem, 19(5), pp. 551-555. (doi: 10.1002/cphc.201800012) (PMID:29316151)
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Abstract
Non-enzymatic DNA strand displacement is an important mechanism in dynamic DNA nanotechnology. Here we show that the large parameter space that is accessible by single-molecule FRET is ideal for the simultaneous monitoring of multiple reactants and products of DNA strand exchange reactions. We monitored the strand displacement from double-stranded DNA (dsDNA) by single-stranded DNA (ssDNA) at 37 °C; the data were modelled as a second-order reaction approaching equilibrium, with a rate constant of ca. 10 M-1 s-1. We also followed the displacement from a DNA three-way junction (3WJ) by ssDNA. The presence of three internal mismatched bases in the middle of the invading strand did not prevent displacement from the 3WJ, but reduced the second-order rate constant by ca. 50 %. We attribute strand exchange in the dsDNA and 3WJ to a zero-toehold pathway from the blunt-ended duplex arms. The single-molecule approach demonstrated here will be useful for studying complex DNA networks.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Magennis, Dr Steven and Morten, Dr Michael and Baltierra Jasso, Dr Laura |
| Authors: | Baltierra Jasso, L., Morten, M., and Magennis, S. W. |
| College/School: | College of Science and Engineering > School of Chemistry |
| Journal Name: | ChemPhysChem |
| Publisher: | Wiley |
| ISSN: | 1439-4235 |
| ISSN (Online): | 1439-7641 |
| Published Online: | 05 January 2018 |
| Copyright Holders: | Copyright © 2018 Wiley-VCH Verlag GmbH & Co. |
| First Published: | First published in ChemPhysChem 19(5):551-555 |
| Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
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