Multiplexing for oxidative bisulfite sequencing (oxBS-seq)

Kirschner, K. , Krueger, F., Green, A. R. and Chandra, T. (2018) Multiplexing for oxidative bisulfite sequencing (oxBS-seq). In: Tost, J. (ed.) DNA Methylation Protocols. Series: Methods in molecular biology, 1708 (1708). Humana Press: New York, pp. 665-678. ISBN 9781493974795 (doi: 10.1007/978-1-4939-7481-8_34)

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DNA modifications, especially methylation, are known to play a crucial part in many regulatory processes in the cell. Recently, 5-hydroxymethylcytosine (5hmC) was discovered, a DNA modification derived as an intermediate of 5-methylcytosine (5mC) oxidation. Efforts to gain insights into function of this DNA modification are underway and several methods were recently described to assess 5hmC levels using sequencing approaches. Here we integrate adaptation based multiplexing and high-efficiency library prep into the oxidative Bisulfite Sequencing (oxBS-seq) workflow reducing the starting amount and cost per sample to identify 5hmC levels genome-wide.

Item Type:Book Sections
Glasgow Author(s) Enlighten ID:Kirschner, Dr Kristina
Authors: Kirschner, K., Krueger, F., Green, A. R., and Chandra, T.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Publisher:Humana Press

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