Tullius, R. et al. (2017) Superchiral plasmonic phase sensitivity for fingerprinting of protein interface structure. ACS Nano, 11(12), pp. 12049-12056. (doi: 10.1021/acsnano.7b04698) (PMID:29220155)
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Abstract
The structure adopted by biomaterials, such as proteins, at interfaces is a crucial parameter in a range of important biological problems. It is a critical property in defining the functionality of cell/bacterial membranes and biofilms (i.e., in antibiotic-resistant infections) and the exploitation of immobilized enzymes in biocatalysis. The intrinsically small quantities of materials at interfaces precludes the application of conventional spectroscopic phenomena routinely used for (bio)structural analysis due to a lack of sensitivity. We show that the interaction of proteins with superchiral fields induces asymmetric changes in retardation phase effects of excited bright and dark modes of a chiral plasmonic nanostructure. Phase retardations are obtained by a simple procedure, which involves fitting the line shape of resonances in the reflectance spectra. These interference effects provide fingerprints that are an incisive probe of the structure of interfacial biomolecules. Using these fingerprints, layers composed of structurally related proteins with differing geometries can be discriminated. Thus, we demonstrate a powerful tool for the bioanalytical toolbox.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Lapthorn, Dr Adrian and Kadodwala, Professor Malcolm and Cooke, Professor Graeme and TULLIUS, Ryan and Karimullah, Dr Affar and Barron, Professor Laurence and Gadegaard, Professor Nikolaj |
| Authors: | Tullius, R., Platt, G. W., Khosravi Khorashad, L., Gadegaard, N., Lapthorn, A. J., Rotello, V. M., Cooke, G., Barron, L. D., Govorov, A. O., Karimullah, A. S., and Kadodwala, M. |
| College/School: | College of Science and Engineering > School of Chemistry College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | ACS Nano |
| Publisher: | American Chemical Society |
| ISSN: | 1936-0851 |
| ISSN (Online): | 1936-086X |
| Published Online: | 08 December 2017 |
| Copyright Holders: | Copyright © 2017 American Chemical Society |
| First Published: | First published in ACS Nano 11(12): 12049-12056 |
| Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
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