Transcriptomic profiling of human breast and melanoma cells selected by migration through narrow constraints

Rudzka, D. A., Clark, W., Hedley, A., Kalna, G. and Olson, M. F. (2017) Transcriptomic profiling of human breast and melanoma cells selected by migration through narrow constraints. Scientific Data, 4, 170172. (doi: 10.1038/sdata.2017.172) (PMID:29135975) (PMCID:PMC5685158)

Abstract

The metastatic spread of cancer cells is a step-wise process that starts with dissociation from primary tumours and local invasion of adjacent tissues. The ability to invade local tissues is the product of several processes, including degradation of extracellular matrices (ECM) and movement of tumour cells through physically-restricting gaps. To identify properties contributing to tumour cells squeezing through narrow gaps, invasive MDA-MB-231 human breast cancer and MDA-MB-435 human melanoma cells were subjected to three successive rounds of selection using cell culture inserts with highly constraining 3 μm pores. For comparison purposes, flow cytometry was also employed to enrich for small diameter MDA-MB-231 cells. RNA-Sequencing (RNA-seq) using the Illumina NextSeq 500 platform was undertaken to characterize how gene expression differed between parental, invasive pore selected or small diameter cells. Gene expression results obtained by RNA-seq were validated by comparing with RT-qPCR. Transcriptomic data generated could be used to determine how alterations that enable cell passage through narrow spaces contribute to local invasion and metastasis.

• Item Type: Articles
• Additional Information: Funding for this project was from Cancer Research UK (A18276).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Clark, Mr William and Kalna, Dr Gabriela and Rudzka, Dominika Agnieszka and Olson, Professor Michael
• Authors: Rudzka, D. A., Clark, W., Hedley, A., Kalna, G., and Olson, M. F.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Scientific Data
• Publisher: Nature Publishing Group
• ISSN: 2052-4463
• ISSN (Online): 2052-4463
• Copyright Holders: Copyright © 2017 The Authors
• First Published: First published in Scientific Data 4: 170172
• Publisher Policy: Reproduced under a Creative Commons License