Evolvability of yeast protein-protein interaction interfaces

Talavera, D., Williams, S. G., Norris, M. G.S., Robertson, D. l. and Lovell, S. C. (2012) Evolvability of yeast protein-protein interaction interfaces. Journal of Molecular Biology, 419(5), pp. 387-96. (doi:10.1016/j.jmb.2012.03.021) (PMID:22472422)

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The functional importance of protein-protein interactions indicates that there should be strong evolutionary constraint on their interaction interfaces. However, binding interfaces are frequently affected by amino acid replacements. Change due to coevolution within interfaces can contribute to variability but is not ubiquitous. An alternative explanation for the ability of surfaces to accept replacements may be that many residues can be changed without affecting the interaction. Candidates for these types of residues are those that make interchain interaction only through the protein main chain, β-carbon, or associated hydrogen atoms. Since almost all residues have these atoms, we hypothesize that this subset of interface residues may be more easily substituted than those that make interactions through other atoms. We term such interactions "residue type independent." Investigating this hypothesis, we find that nearly a quarter of residues in protein interaction interfaces make exclusively interchain residue-type-independent contacts. These residues are less structurally constrained and less conserved than residues making residue-type-specific interactions. We propose that residue-type-independent interactions allow substitutions in binding interfaces while the specificity of binding is maintained.

Item Type:Articles
Additional Information:This work was supported by Biotechnology and Biological Sciences Research Council grants BB/ F013337/1 and BB/F007620/1.
Glasgow Author(s) Enlighten ID:Robertson, Professor David
Authors: Talavera, D., Williams, S. G., Norris, M. G.S., Robertson, D. l., and Lovell, S. C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Molecular Biology
ISSN (Online):1089-8638

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