The development of Dirofilaria immitis in cultured malpighian tubules

Devaney, E. (1981) The development of Dirofilaria immitis in cultured malpighian tubules. Acta Tropica, 38(3), pp. 251-60. (PMID:6118033)

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A technique is described for the cultivation in vitro of Dirofilaria immitis to the second larval stage within malpighian tubules excised from mosquitoes 20-24 h post infection. The malpighian tubules were removed from mosquitoes under aseptic conditions, washed and inoculated into a variety of tissue culture media, both insect and mammalian. In some cultures a feeder layer of Aedes malayensis cells was included. The 24 h larvae developed to the second stage only in Schneider's Drosophila medium containing 20% foetal bovine serum, irrespective of whether a cell layer was included in the cultures. Development of the larvae to the infective third stage was not observed.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Devaney, Professor Eileen
Authors: Devaney, E.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Acta Tropica

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