Dirofilaria immitis: the moulting of the infective larva in vitro.

Devaney, E. (1985) Dirofilaria immitis: the moulting of the infective larva in vitro. Journal of Helminthology, 59(1), pp. 47-50. (PMID:3989262)

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A number of in vitro culture systems were tested for their ability to support the development of Dirofilaria immitis infective larvae to the fourth larval stage. In cultures of medium ML-15 containing a feeder layer of Dog Sarcoma (DS) cells larvae successfully moulted and showed a small but significant increase in length. Ultrastructural observations demonstrated that the fourth-stage cuticle was synthesized in vitro and in some larvae was fully formed by 60 hours of culture. The hypodermis of moulting larvae contained numerous multi-vesicular bodies. It is concluded that the moult in vitro is a true moult and not an atypical response of the larvae to the conditions of culture.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Devaney, Professor Eileen
Authors: Devaney, E.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Journal of Helminthology
Publisher:Cambridge University Press
ISSN (Online):1475-2697

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