Bovis, M.J. et al. (2015) Photosensitisation studies of silicone polymer doped with methylene blue and nanogold for antimicrobial applications. RSC Advances, 5(68), pp. 54830-54842. (doi: 10.1039/c5ra09045h)
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Abstract
Photosensitisation of polymers has important potential clinical applications such as the prevention of catheter-associated urinary tract infections (CAUTIs). Polymers incorporated with methylene blue (MB) and 2 nm gold nanoparticles (AuNPs) are effective in killing bacteria at the surface following low power visible illumination. Studies of medical-grade silicone polymer samples including segments from urinary catheters were carried out to investigate the generation of reactive oxygen species and the involvement of Type 1 and 2 mechanisms. Singlet oxygen was observed using direct phosphorescence detection and hydroxyl radical generation using electron paramagnetic resonance (EPR) spectroscopy; we conclude that both Type 1 and 2 mechanisms can operate with polymeric photosensitisation. Transmission electron microscopy (TEM) directly demonstrated the incorporation of AuNPs at the surface of the silicone. Using silicone doped with MB AuNPs, a ≥3 log10 reduction in the number of viable Staphylococcus epidermidis bacteria was achieved when exposed to low power laser light; prior sterilisation with ethylene oxide (EO) had no influence on efficacy.
Item Type: | Articles |
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Additional Information: | This work was supported by the Medical Research Council. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Peveler, Dr William |
Authors: | Bovis, M.J., Noimark, S., Woodhams, J.H., Kay, C.W.M., Weiner, J., Peveler, W.J., Correia, A., Wilson, M., Allan, E., Parkin, I.P., and MacRobert, A.J. |
College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
Journal Name: | RSC Advances |
Publisher: | Royal Society of Chemistry |
ISSN: | 2046-2069 |
ISSN (Online): | 2046-2069 |
Published Online: | 12 June 2015 |
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