Plasmonic nanoprobes for stimulated emission depletion nanoscopy

Cortes, E. et al. (2016) Plasmonic nanoprobes for stimulated emission depletion nanoscopy. ACS Nano, 10(11), pp. 10454-10461. (doi: 10.1021/acsnano.6b06361) (PMID:27794591)

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Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles in order to enhance the stimulated depletion of excited molecules for super-resolved nanoscopy. We demonstrate stimulated emission depletion (STED) nanoscopy with gold nanorods with a long axis of only 26 nm and a width of 8 nm. These particles provide an enhancement of up to 50% of the resolution compared to fluorescent-only probes without plasmonic components irradiated with the same depletion power. The nanoparticle-assisted STED probes reported here represent a ∼2 × 103 reduction in probe volume compared to previously used nanoparticles. Finally, we demonstrate their application toward plasmon-assisted STED cellular imaging at low-depletion powers, and we also discuss their current limitations.

Item Type:Articles
Additional Information:This project was supported by the MRC Next Generation Optical Microscopy Initiative, (MR/K015834/1), EPSRC Reactive Plasmonics project EP/M013812/1, the Royal Society, and the Leverhulme Trust. E.C. and P.A.H. are supported by Marie-Sklodowska Curie fellowships. W.P. is supported by an EPSRC Doctoral Prize Fellowship (EP/ M506448/1). H.S. acknowledges a CASE Ph.D. studentship from the U.K. Engineering and Physical Sciences Research Council (EPSRC) and the Diamond Trading Company. Y.S. acknowledges the support from the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement no. 333790 as well as the support of the Alumni program of the International Newton Fellowship program of the Royal Society and of the Israeli national nanotechnology Initiative. S.A.M. acknowledgers the Lee-Lucas Chair in Physics.
Glasgow Author(s) Enlighten ID:Peveler, Dr William
Authors: Cortes, E., Huidobro, P. A., Sinclair, H. G., Guldbrand, S., Peveler, W. J., Davies, T., Parrinello, S., Gorlitz, F., Dunsby, C., Neil, M. A.A., Sivan, Y., Parkin, I. P., French, P. M.W., and Maier, S. A.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:ACS Nano
Publisher:American Chemical Society
ISSN (Online):1936-086X
Published Online:30 October 2016
Copyright Holders:Copyright © 2016 American Chemical Society
First Published:First published in ACS Nano 10(11): 10454-10461
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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