Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8

Walden, H. , Podgorski, M. S. and Schulman, B. A. (2003) Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8. Nature, 422(6929), pp. 330-334. (doi: 10.1038/nature01456) (PMID:12646924)

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Post-translational modification by ubiquitin-like proteins (Ublps) is an essential cellular regulatory mechanism1, 2, 3. The Ublp NEDD8 regulates cell division, signalling and embryogenesis4, 5, 6. Ublps are conjugated to their targets by the sequential action of E1, E2 and often E3 enzymes3. Each Ublp has a dedicated E1, or activating enzyme, that initiates its conjugation cascade1, 3, 7, 8, 9, 10. First, E1 associates with the Ublp and catalyses adenylation of the carboxy terminus of the Ublp. Second, E1 forms a thioester between its catalytic cysteine and the Ublp. Next, E1 is loaded with a second Ublp molecule, adenylating the C terminus of this second Ublp while still carrying the first thioester-bound Ublp. Last, E1 binds E2 and promotes Ublp transfer to the catalytic cysteine of E2. We report here the structure and mutational analysis of human APPBP1–UBA3, the heterodimeric E1 enzyme for NEDD8 (ref. 11). Each E1 activity is specified by a domain: an adenylation domain resembling bacterial adenylating enzymes12, an E1-specific domain organized around the catalytic cysteine, and a domain involved in E2 recognition resembling ubiquitin. The domains are arranged around two clefts that coordinate protein and nucleotide binding so that each of E1's reactions drives the next, in an assembly-line fashion.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Walden, Professor Helen
Authors: Walden, H., Podgorski, M. S., and Schulman, B. A.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Journal Name:Nature
Publisher:Nature Publishing Group
ISSN (Online):1476-4687

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