Selenium incorporation using recombinant techniques

Walden, H. (2010) Selenium incorporation using recombinant techniques. Acta Crystallographica. Section D: Biological Crystallography, 66(4), pp. 352-357. (doi: 10.1107/S0907444909038207) (PMID:20382987) (PMCID:PMC2852298)

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Using selenomethionine to phase macromolecular structures is common practice in structure determination, along with the use of selenocysteine. Selenium is consequently the most commonly used heavy atom for MAD. In addition to the well established recombinant techniques for the incorporation of selenium in pro­karyal expression systems, there have been recent advances in selenium labelling in eukaryal expression, which will be discussed. Tips and things to consider for the purification and crystallization of seleno-labelled proteins are also included.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Walden, Professor Helen
Authors: Walden, H.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Journal Name:Acta Crystallographica. Section D: Biological Crystallography
Publisher:Wiley-Blackwell Publishing, Inc.
ISSN (Online):1399-0047
Copyright Holders:Copyright © 2010 The Author
First Published:First published in Acta Crystallographica. Section D: Biological Crystallography 66(4):352-357
Publisher Policy:Reproduced under a Creative Commons License

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