Walden, H. (2010) Selenium incorporation using recombinant techniques. Acta Crystallographica. Section D: Biological Crystallography, 66(4), pp. 352-357. (doi: 10.1107/S0907444909038207) (PMID:20382987) (PMCID:PMC2852298)
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Abstract
Using selenomethionine to phase macromolecular structures is common practice in structure determination, along with the use of selenocysteine. Selenium is consequently the most commonly used heavy atom for MAD. In addition to the well established recombinant techniques for the incorporation of selenium in prokaryal expression systems, there have been recent advances in selenium labelling in eukaryal expression, which will be discussed. Tips and things to consider for the purification and crystallization of seleno-labelled proteins are also included.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Walden, Professor Helen |
Authors: | Walden, H. |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Acta Crystallographica. Section D: Biological Crystallography |
Publisher: | Wiley-Blackwell Publishing, Inc. |
ISSN: | 0907-4449 |
ISSN (Online): | 1399-0047 |
Copyright Holders: | Copyright © 2010 The Author |
First Published: | First published in Acta Crystallographica. Section D: Biological Crystallography 66(4):352-357 |
Publisher Policy: | Reproduced under a Creative Commons License |
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