A molecular explanation for the recessive nature of parkin-linked Parkinson’s disease

Spratt, D. E. et al. (2013) A molecular explanation for the recessive nature of parkin-linked Parkinson’s disease. Nature Communications, 4, 1983. (doi: 10.1038/ncomms2983) (PMID:23770917) (PMCID:PMC3709501)

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Mutations in the park2 gene, encoding the RING-inBetweenRING-RING E3 ubiquitin ligase parkin, cause 50% of autosomal recessive juvenile Parkinsonism cases. More than 70 known pathogenic mutations occur throughout parkin, many of which cluster in the inhibitory amino-terminal ubiquitin-like domain, and the carboxy-terminal RING2 domain that is indispensable for ubiquitin transfer. A structural rationale showing how autosomal recessive juvenile Parkinsonism mutations alter parkin function is still lacking. Here we show that the structure of parkin RING2 is distinct from canonical RING E3 ligases and lacks key elements required for E2-conjugating enzyme recruitment. Several pathogenic mutations in RING2 alter the environment of a single surface-exposed catalytic cysteine to inhibit ubiquitination. Native parkin adopts a globular inhibited conformation in solution facilitated by the association of the ubiquitin-like domain with the RING-inBetweenRING-RING C-terminus. Autosomal recessive juvenile Parkinsonism mutations disrupt this conformation. Finally, parkin autoubiquitinates only in cis, providing a molecular explanation for the recessive nature of autosomal recessive juvenile Parkinsonism.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Walden, Professor Helen
Authors: Spratt, D. E., Julio Martinez-Torres, R., Noh, Y. J., Mercier, P., Manczyk, N., Barber, K. R., Aguirre, J. D., Burchell, L., Purkiss, A., Walden, H., and Shaw, G. S.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Journal Name:Nature Communications
Publisher:Nature Publishing Group
ISSN (Online):2041-1723
Copyright Holders:Copyright © 2013 The Authors
First Published:First published in Nature Communications 4:1983
Publisher Policy:Reproduced under a Creative Commons License

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