Mallo, N., DeFelipe, A.P., Folgueira, I., Sueiro, R.A., Lamas, J. and Leiro, J.M. (2017) Combined antiparasitic and anti-inflammatory effects of the natural polyphenol curcumin on turbot scuticociliatosis. Journal of Fish Diseases, 40(2), pp. 205-217. (doi: 10.1111/jfd.12503) (PMID:27334368)
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Abstract
The histiophagous scuticociliate Philasterides dicentrarchi is the aetiological agent of scuticociliatosis, a parasitic disease of farmed turbot. Curcumin, a polyphenol from Curcuma longa (turmeric), is known to have antioxidant and anti-inflammatory properties. We investigated the in vitro effects of curcumin on the growth of P. dicentrarchi and on the production of pro-inflammatory cytokines in turbot leucocytes activated by parasite cysteine proteases. At 100 μm, curcumin had a cytotoxic effect and completely inhibited the growth of the parasite. At 50 μm, curcumin inhibited the protease activity of the parasite and expression of genes encoding two virulence-associated proteases: leishmanolysin-like peptidase and cathepsin L-like. At concentrations between 25 and 50 μm, curcumin inhibited the expression of S-adenosyl-L-homocysteine hydrolase, an enzyme involved in the biosynthesis of the amino acids methionine and cysteine. At 100 μm, curcumin inhibited the expression of the cytokines tumour necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) produced in turbot leucocytes activated by parasite proteases. Results show that curcumin has a dual effect on scuticociliatosis: an antiparasitic effect on the catabolism and anabolism of ciliate proteins, and an anti-inflammatory effect that inhibits the production of proinflammatory cytokines in the host. The present findings suggest the potential usefulness of this polyphenol in treating scuticociliatosis.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was financially supported by the Minis-terio de Economıa y Competitividad (Spain)under grant agreement AGL2014-57125-R, by theEuropean Union’s Horizon 2020 research andinnovation programme under grant agreementNo. 634429 (PARAFISHC ONTROL) and by theXunta de Galicia (Spain) under grant agreementGPC2014/069. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Mallo, Dr Natalia |
| Authors: | Mallo, N., DeFelipe, A.P., Folgueira, I., Sueiro, R.A., Lamas, J., and Leiro, J.M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Journal of Fish Diseases |
| Publisher: | Wiley |
| ISSN: | 0140-7775 |
| ISSN (Online): | 1365-2761 |
| Published Online: | 23 June 2016 |
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