Tsai, I. J., Otto, T. D. and Berriman, M. (2010) Improving draft assemblies by iterative mapping and assembly of short reads to eliminate gaps. Genome Biology, 11, R41. (doi: 10.1186/gb-2010-11-4-r41) (PMID:20388197) (PMCID:PMC2884544)
|
Text
148164.pdf - Published Version Available under License Creative Commons Attribution. 1MB |
Abstract
Advances in sequencing technology allow genomes to be sequenced at vastly decreased costs. However, the assembled data frequently are highly fragmented with many gaps. We present a practical approach that uses Illumina sequences to improve draft genome assemblies by aligning sequences against contig ends and performing local assemblies to produce gap-spanning contigs. The continuity of a draft genome can thus be substantially improved, often without the need to generate new data.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Otto, Professor Thomas |
| Authors: | Tsai, I. J., Otto, T. D., and Berriman, M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Genome Biology |
| Publisher: | BioMed Central |
| ISSN: | 1465-6906 |
| ISSN (Online): | 1465-6906 |
| Copyright Holders: | Copyright ©2010 Tsai et al. |
| First Published: | First published in Genome Biology 11:R41 |
| Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record
Deposit and Record Details
Deposit and Record Details