Pediani, J. D. , Ward, R. J., Marsango, S. and Milligan, G. (2018) Spatial intensity distribution analysis: studies of G Protein-coupled receptor oligomerization. Trends in Pharmacological Sciences, 39(2), pp. 175-186. (doi: 10.1016/j.tips.2017.09.001) (PMID:29032835) (PMCID:PMC5783713)
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Abstract
Spatial intensity distribution analysis (SpIDA) is a recently developed approach for determining quaternary structure information on fluorophore-labelled proteins of interest in situ. It can be applied to live or fixed cells and native tissue. Using confocal images, SpIDA generates fluorescence intensity histograms that are analysed by super-Poissonian distribution functions to obtain density and quantal brightness values of the fluorophore-labelled protein of interest. This allows both expression level and oligomerisation state of the protein to be determined. We describe the application of SpIDA to investigate the oligomeric state of G protein-coupled receptors (GPCRs) at steady state and following cellular challenge, and consider how SpIDA may be used to explore GPCR quaternary organisation in pathophysiology and to stratify medicines.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Pediani, Dr John and Ward, Dr Richard and Milligan, Professor Graeme and Marsango, Dr Sara |
Authors: | Pediani, J. D., Ward, R. J., Marsango, S., and Milligan, G. |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Trends in Pharmacological Sciences |
Publisher: | Elsevier |
ISSN: | 0165-6147 |
ISSN (Online): | 1873-3735 |
Published Online: | 06 October 2017 |
Copyright Holders: | Copyright © 2017 The Authors |
First Published: | First published in Trends in Pharmacological Sciences 39(2):175-186 |
Publisher Policy: | Reproduced under a Creative Commons License |
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