Yuan, X. , Couto, J. M., Glidle, A., Song, Y. , Sloan, W. and Yin, H. (2017) Single-cell microfluidics to study the effects of genome deletion on bacterial growth behavior. ACS Synthetic Biology, 6(12), pp. 2219-2227. (doi: 10.1021/acssynbio.7b00177) (PMID:28844132)
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Abstract
By directly monitoring single cell growth in a microfluidic platform, we interrogated genome-deletion effects in Escherichia coli strains. We compared the growth dynamics of a wild type strain with a clean genome strain, and their derived mutants at the single-cell level. A decreased average growth rate and extended average lag time were found for the clean genome strain, compared to those of the wild type strain. Direct correlation between the growth rate and lag time of individual cells showed that the clean genome population was more heterogeneous. Cell culturability (the ratio of growing cells to the sum of growing and nongrowing cells) of the clean genome population was also lower. Interestingly, after the random mutations induced by a glucose starvation treatment, for the clean genome population mutants that had survived the competition of chemostat culture, each parameter markedly improved (i.e., the average growth rate and cell culturability increased, and the lag time and heterogeneity decreased). However, this effect was not seen in the wild type strain; the wild type mutants cultured in a chemostat retained a high diversity of growth phenotypes. These results suggest that quasi-essential genes that were deleted in the clean genome might be required to retain a diversity of growth characteristics at the individual cell level under environmental stress. These observations highlight that single-cell microfluidics can reveal subtle individual cellular responses, enabling in-depth understanding of the population.
| Item Type: | Articles |
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| Keywords: | Cell growth, genome reduction, microfluidics, mutation, single-cell. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Yin, Professor Huabing and Song, Dr Yanqing and Sloan, Professor William and Couto, Dr Jillian and Yuan, Dr Xiaofei and Glidle, Dr Andrew |
| Authors: | Yuan, X., Couto, J. M., Glidle, A., Song, Y., Sloan, W., and Yin, H. |
| College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering College of Science and Engineering > School of Engineering > Infrastructure and Environment |
| Journal Name: | ACS Synthetic Biology |
| Publisher: | American Chemical Society |
| ISSN: | 2161-5063 |
| ISSN (Online): | 2161-5063 |
| Published Online: | 26 August 2017 |
| Copyright Holders: | Copyright © 2017 American Chemical Society |
| First Published: | First published in ACS Synthetic Biology 6(12): 2219-2227 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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