Wang, Y., Dacosta, C., Wang, W., Zhou, Z., Liu, M. and Bao, Y. (2015) Synergy between sulforaphane and selenium in protection against oxidative damage in colonic CCD841 cells. Nutrition Research, 35(7), pp. 610-617. (doi: 10.1016/j.nutres.2015.05.011) (PMID:26094214)
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Abstract
Dietary isothiocyanates are potent inducers of the NF-E2-related factor 2 (Nrf2) pathway. Sulforaphane (SFN), a representative dietary isothiocyanate, has previously been shown to up-regulate antioxidant enzymes such as selenium (Se)-dependent thioredoxin reductase-1 (TrxR-1) in many tumor cell lines. In the present study, we hypothesized that a combination of SFN and Se would have a synergistic effect on the up-regulation of TrxR-1 and the protection against oxidative damage in the normal colonic cell line CCD841. Treatment of cells with SFN and Se significantly induced TrxR-1 expression. Pretreatment of cells with SFN protects against H2O2-induced cell death; this protection was enhanced by cotreatment with Se. The small interfering RNA knockdown of either TrxR-1 or Nrf2 reduced the protection afforded by SFN and Se cotreatment; TrxR-1 and Nrf2 knockdown reduced cell viabilities to 66.5% and 51.1%, respectively, down from 82.4% in transfection-negative controls. This suggests that both TrxR-1 and Nrf2 are important in SFN-mediated protection against free radical-induced cell death. Moreover, flow cytometric analysis showed that TrxR-1 and Nrf2 were involved in SFN-mediated protection against H2O2-induced apoptosis. In summary, SFN activates the Nrf2 signaling pathway and protects against H2O2-mediated oxidative damage in normal colonic cells. Combined SFN and Se treatment resulted in a synergistic up-regulation of TrxR-1 that in part contributed to the enhanced protection against free radical-mediated cell death provided by the cotreatment.
Item Type: | Articles |
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Keywords: | Cruciferous vegetable, sulforaphane, selenium, thioredoxin reductase, Nrf2, colon cancer. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Dacosta, Dr Christopher |
Authors: | Wang, Y., Dacosta, C., Wang, W., Zhou, Z., Liu, M., and Bao, Y. |
College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
Journal Name: | Nutrition Research |
Journal Abbr.: | Nutr Res. |
Publisher: | Elsevier |
ISSN: | 0271-5317 |
ISSN (Online): | 1879-0739 |
Published Online: | 30 May 2015 |
Copyright Holders: | Copyright © 2015 Elsevier Inc. |
First Published: | First published in Nutrition Research 35(7): 610-617 |
Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
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