Neutrophil-derived microparticles are released into the coronary circulation following percutaneous coronary intervention in acute coronary syndrome patients

Martínez, G. J., Barraclough, J. Y., Nakhla, S., Kienzle, V., Robertson, S., Mallat, Z., Celermajer, D. S. and Patel, S. (2017) Neutrophil-derived microparticles are released into the coronary circulation following percutaneous coronary intervention in acute coronary syndrome patients. Bioscience Reports, 37(1), BSR2016043. (doi: 10.1042/BSR20160430) (PMID:27913753) (PMCID:PMC5240586)

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Abstract

To evaluate (i) local coronary and systemic levels of microparticles (MP) in acute coronary syndrome (ACS) and stable angina pectoris (SAP) patients and (ii) their release after plaque disruption with percutaneous coronary intervention (PCI). MP are small vesicles originating from plasma membranes of cells after activation or apoptosis and are implicated in the pathogenesis of atherosclerosis. Neutrophils play a role in plaque destabilization and shed neutrophil-derived MP that have the potential to drive significant proinflammatory and thrombotic downstream effects. Eight ACS and eight SAP patients were included. Coronary sinus (CS) samples pre-intervention (CS1), 45 s following balloon angioplasty (CS2) and at 45 s intervals following stent deployment (CS3, CS4 and CS5), together with peripheral vein samples, pre- and post-PCI were analysed for neutrophil-derived (CD66b+), endothelial-derived (CD144+), platelet-derived (CD41a+), monocyte-derived (CD14+) and apoptotic (Annexin V+) MP. ELISA for interleukin (IL)-6, myeloperoxidase (MPO) and P-selectin was also performed. CD66b+ MP levels were similar in both groups pre-intervention. Post-PCI, CS levels rose significantly in ACS but not SAP patients (ACS area under the curve (AUC): 549 +− 83, SAP AUC: 24 +− 29, P<0.01). CS CD41a+, CD144+, CD14+ and Annexin V+ MP levels did not differ between groups. Acute neutrophil-derived MP release post-PCI occurs in ACS compared with stable patients, likely to be reflective of plaque MP content in vulnerable lesions.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Robertson, Dr Stacy
Authors: Martínez, G. J., Barraclough, J. Y., Nakhla, S., Kienzle, V., Robertson, S., Mallat, Z., Celermajer, D. S., and Patel, S.
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
Journal Name:Bioscience Reports
Publisher:Portland Press
ISSN:0144-8463
ISSN (Online):1573-4935
Published Online:17 January 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Bioscience Reports 37(1): BSR2016043
Publisher Policy:Reproduced under a Creative Commons license

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