Lee, A. V., Atkinson, C., Manuel, R. J. and Clark, D. A. (2011) Comparative evaluation of the QIAGEN QIAsymphony® SP system and bioMérieux NucliSens easyMAG automated extraction platforms in a clinical virology laboratory. Journal of Clinical Virology, 52(4), pp. 339-343. (doi: 10.1016/j.jcv.2011.08.016) (PMID:22014618)
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Abstract
Background: The QIAGEN QIAsymphony® SP is an automated system that can process a variety of different sample types for nucleic acid extraction. Objectives: To compare this system against the bioMérieux NucliSens easyMAG using a range of common clinical sample types. Study design: Nucleic acid extracts were tested on 6 in-house real time viral PCR assays: quantitative cytomegalovirus (CMV); respiratory and enteric multiplex (10 viruses); influenza A H1N1 2009 specific H1 and N1 assays (sH1/N1); herpes simplex virus (HSV) 1, HSV 2 and varicella zoster virus (VZV) multiplex; norovirus genogroups I and II (Noro GI/II) multiplex. Results: Extraction of the clinical sample by either QIAsymphony® SP or NucliSens easyMAG gave similar results for each PCR; CMV viral loads, 52 plasma samples had a mean difference (easyMAG–QIAsymphony®) of 0.002 log10 copies/ml (s.d. 0.536), 52 whole blood samples had a mean difference of −0.232 log10 copies/ml (s.d. 0.490). Concordance for the qualitative assays were; 64/67 (95.5%) for the respiratory and enteric multiplex, all 28 (100%) for the sH1, sN1 and influenza A matrix multiplex, 33/34 (97%) for the HSV1/HSV2/VZV multiplex and all 15 (100%) for the Noro GI/II. Inter- and intra-run variation, determined for a 10-fold dilution series of CMV (5.20–3.20 log10 copies/ml), was less than 0.63 log10 copies/ml. Conclusions: Our evaluation found the performance of the QIAsymphony® SP comparable to the NucliSens easyMAG for a range of sample types commonly extracted in a clinical virology laboratory. In total, 331/343 (96.5%) PCR results were concordant on samples extracted by both platforms.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Clark, Dr Duncan |
| Authors: | Lee, A. V., Atkinson, C., Manuel, R. J., and Clark, D. A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Life Sciences |
| Journal Name: | Journal of Clinical Virology |
| Publisher: | Elsevier |
| ISSN: | 1873-5967 |
| ISSN (Online): | 1386-6532 |
| Published Online: | 19 October 2011 |
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