Monitoring genetic population biomarkers for wastewater-based epidemiology

Yang, Z. , Xu, G., Reboud, J. , Kasprzyk-Hordern, B. and Cooper, J. M. (2017) Monitoring genetic population biomarkers for wastewater-based epidemiology. Analytical Chemistry, 89(18), 9941–9945-9941–9945. (doi: 10.1021/acs.analchem.7b02257) (PMID:28814081)

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We report a rapid “sample-to-answer” platform that can be used for the quantitative monitoring of genetic biomarkers within communities through the analysis of wastewater. The assay is based on the loop-mediated isothermal amplification (LAMP) of nucleic acid biomarkers and shows for the first time the ability to rapidly quantify human-specific mitochondrial DNA (mtDNA) from raw untreated wastewater samples. mtDNA provides a model population biomarker associated with carcinogenesis including breast, renal and gastric cancers. To enable a sample-to-answer, field-based technology, we integrated a filter to remove solid impurities and perform DNA extraction and enrichment into a low cost lateral flow-based test. We demonstrated mtDNA detection over seven consecutive days, achieving a limit of detection of 40 copies of human genomic DNA per reaction volume. The assay can be performed at the site of sample collection, with minimal user intervention, yielding results within 45 min and providing a method to monitor public health from wastewater.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan and Reboud, Professor Julien and Xu, Mr Gaolian and Yang, Dr Zhugen
Authors: Yang, Z., Xu, G., Reboud, J., Kasprzyk-Hordern, B., and Cooper, J. M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Analytical Chemistry
Publisher:American Chemical Society
ISSN (Online):1520-6882
Published Online:17 August 2017
Copyright Holders:Copyright © 2017 American Chemical Society
First Published:First published in Analytical Chemistry 89(18):9941–9945
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
617021Advanced Diagnostics using PhononicsJonathan CooperEngineering and Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - BIOMEDICAL ENGINEERING
634061Development of multiplexed diagnostic biosensor for infectious reproductive diseases of cattle and buffaloesJonathan CooperBiotechnology and Biological Sciences Research Council (BBSRC)BB/L00464X/1ENG - BIOMEDICAL ENGINEERING