Yang, Z. , Xu, G., Reboud, J. , Kasprzyk-Hordern, B. and Cooper, J. M. (2017) Monitoring genetic population biomarkers for wastewater-based epidemiology. Analytical Chemistry, 89(18), 9941–9945-9941–9945. (doi: 10.1021/acs.analchem.7b02257) (PMID:28814081)
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Abstract
We report a rapid “sample-to-answer” platform that can be used for the quantitative monitoring of genetic biomarkers within communities through the analysis of wastewater. The assay is based on the loop-mediated isothermal amplification (LAMP) of nucleic acid biomarkers and shows for the first time the ability to rapidly quantify human-specific mitochondrial DNA (mtDNA) from raw untreated wastewater samples. mtDNA provides a model population biomarker associated with carcinogenesis including breast, renal and gastric cancers. To enable a sample-to-answer, field-based technology, we integrated a filter to remove solid impurities and perform DNA extraction and enrichment into a low cost lateral flow-based test. We demonstrated mtDNA detection over seven consecutive days, achieving a limit of detection of 40 copies of human genomic DNA per reaction volume. The assay can be performed at the site of sample collection, with minimal user intervention, yielding results within 45 min and providing a method to monitor public health from wastewater.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cooper, Professor Jonathan and Reboud, Dr Julien and Xu, Mr Gaolian and Yang, Dr Zhugen |
| Authors: | Yang, Z., Xu, G., Reboud, J., Kasprzyk-Hordern, B., and Cooper, J. M. |
| College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Analytical Chemistry |
| Publisher: | American Chemical Society |
| ISSN: | 0003-2700 |
| ISSN (Online): | 1520-6882 |
| Published Online: | 17 August 2017 |
| Copyright Holders: | Copyright © 2017 American Chemical Society |
| First Published: | First published in Analytical Chemistry 89(18):9941–9945 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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