A method of narcotizing snails (Helix pomatia) and cannulating the haemocoel and its application to a study of the role of calcium in the regulation of acid-base balance

Burton, R.F. (1975) A method of narcotizing snails (Helix pomatia) and cannulating the haemocoel and its application to a study of the role of calcium in the regulation of acid-base balance. Comparative Biochemistry and Physiology Part A: Physiology, 52(3), pp. 483-485. (doi: 10.1016/S0300-9629(75)80069-7) (PMID:241546)

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Abstract

1. Cannulae were inserted into the tentacles of snails briefly narcotized with succinylcholine. The resulting acid-base disturbances were temporary. 2. Exposure of snails to 10% CO2 caused the haemolymph concentrations of HCO3 and Ca to rise in 2:1 ratio. Na and Mg were little affected. 3. Infusion of NaHCO3 led to subsequent loss of HCO3 and Ca from the haemolymph in more than 2:1 ratio. Infusion of CaCl2 led to subsequent loss of HCO3 and Ca in a ratio of 2:1 or less.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burton, Dr Richard
Authors: Burton, R.F.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Comparative Biochemistry and Physiology Part A: Physiology
Publisher:Pergamon Press
ISSN:0300-9629

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