Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor

Tsimbouri, P. M. et al. (2017) Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor. Nature Biomedical Engineering, 1, pp. 758-770. (doi: 10.1038/s41551-017-0127-4) (PMID:31015671)

Abstract

Bone grafts are one of the most commonly transplanted tissues. However, autologous grafts are in short supply, and can be associated with pain and donor-site morbidity. The creation of tissue-engineered bone grafts could help to fulfil clinical demand and provide a crucial resource for drug screening. Here, we show that vibrations of nanoscale amplitude provided by a newly developed bioreactor can differentiate a potential autologous cell source, mesenchymal stem cells (MSCs), into mineralized tissue in 3D. We demonstrate that nanoscale mechanotransduction can stimulate osteogenesis independently of other environmental factors, such as matrix rigidity. We show this by generating mineralized matrix from MSCs seeded in collagen gels with stiffness an order of magnitude below the stiffness of gels needed to induce bone formation in vitro. Our approach is scalable and can be compatible with 3D scaffolds.

• Item Type: Articles
• Additional Information: Also funded by EP/N012631/1 to UWS
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Orapiriyakul, Wich and Curtis, Professor Adam and Tsimbouri, Dr Monica and Salmeron-Sanchez, Professor Manuel and Dalby, Professor Matthew and Gonzalez Garcia, Dr Cristina and Blackburn, Dr Gavin and Burgess, Dr Karl and Jayawarna, Dr Vineetha and Childs, Dr Peter and Yang, Dr Jingli
• Authors: Tsimbouri, P. M., Childs, P. G., Pemberton, G. D., Yang, J., Jayawarna, V., Oripiriyakul, W., Burgess, K., Gonzalez-Garcia, C., Blackburn, G., Thomas, D., Giraldo, C. V., Biggs, M. J.P., Curtis, A. S.G., Salmeron-Sanchez, M., Reid, S., and Dalby, M. J.
• College/School: College of Medical Veterinary and Life Sciences; College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Molecular Biosciences; College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Nature Biomedical Engineering
• Publisher: Nature Publishing Group
• ISSN: 2157-846X
• ISSN (Online): 2157-846X
• Copyright Holders: Copyright © 2017 Macmillan Publishers Limited, part of Springer Nature
• First Published: First published in Nature Biomedical Engineering 1:758-770
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher

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Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
70998	1	Development of NanoKick Bioreactor	Matthew Dalby	Biotechnology and Biological Sciences Research Council (BBSRC)	BB/N012690/1	RI MOLECULAR CELL & SYSTEMS BIOLOGY
72838	1	Rapid Bone Graft Synthesis Through Dual Piezoelectric/Nanomechaniocal Stimulation	Matthew Dalby	Biotechnology and Biological Sciences Research Council (BBSRC)	BB/P00220X/1	RI MOLECULAR CELL & SYSTEMS BIOLOGY
69189	1	Developing the NanoKick bioreactor to enable tissue engineered bone graft and use of metabolomics to identify bone specific drug candidates	Matthew Dalby	Engineering and Physical Sciences Research Council (EPSRC)	EP/N013905/1	RI MOLECULAR CELL & SYSTEMS BIOLOGY
72206	1	Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicine	Manuel Salmeron-Sanchez	Engineering and Physical Sciences Research Council (EPSRC)	EP/P001114/1	ENG - BIOMEDICAL ENGINEERING