Watts, A. E. et al. (2017) MicroRNA29a treatment improves early tendon injury. Molecular Therapy, 25(10), pp. 2415-2426. (doi: 10.1016/j.ymthe.2017.07.015) (PMID:28822690)
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Abstract
Tendon injuries (tendinopathies) are common in human and equine athletes and characterised by dysregulated collagen matrix resulting in tendon damage. We have previously demonstrated a functional role for miR29a as a post-transcriptional regulator of collagen 3 expression in murine and human tendon injury. Given the translational potential, we designed a randomised, blinded trial to evaluate the potential of a miR29a replacement therapy as a therapeutic option to treat tendinopathy in an equine model which closely mimics human disease. Tendon injury was induced in the superficial digital flexor tendon (SDFT) of 17 horses. Tendon lesions were treated one week later with an intralesional injection of miR29a or placebo. miR29a treatment reduced collagen 3 transcript levels at week 2 with no significant changes in collagen 1. Relative lesion cross-sectional area was significantly lower in miR29a tendons compared to control tendons. Histology scores were significantly better for miR29a treated tendons compared to control tendons. This data supports the mechanism of miRNA-mediated modulation of the early pathophysiologic events that facilitate tissue remodelling in the tendon after injury and provides strong proof of principle that a locally delivered miR29a therapy improves early tendon healing.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | McInnes, Professor Iain and Akbar, Mr Moeed and Kitson, Miss Susan and Gilchrist, Dr Derek and Millar, Professor Neal |
| Authors: | Watts, A. E., Millar, N. L., Platt, J., Kitson, S. M., Akbar, M., Rech, R., Griffin, J., Pool, R., Hughes, T., McInnes, I. B., and Gilchrist, D.S. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Molecular Therapy |
| Publisher: | Elsevier |
| ISSN: | 1525-0016 |
| ISSN (Online): | 1525-0024 |
| Published Online: | 28 July 2017 |
| Copyright Holders: | Copyright © 2017 The American Society of Gene and Cell Therapy |
| First Published: | First published in Molecular Therapy 25(10): 2415-2426 |
| Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
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