The cancer-associated cell migration protein TSPAN1 is under control of androgens and its upregulation increases prostate cancer cell migration

Munkley, J. et al. (2017) The cancer-associated cell migration protein TSPAN1 is under control of androgens and its upregulation increases prostate cancer cell migration. Scientific Reports, 7, 5249. (doi: 10.1038/s41598-017-05489-5) (PMID:28701765) (PMCID:PMC5507901)

Abstract

Cell migration drives cell invasion and metastatic progression in prostate cancer and is a major cause of mortality and morbidity. However the mechanisms driving cell migration in prostate cancer patients are not fully understood. We previously identified the cancer-associated cell migration protein Tetraspanin 1 (TSPAN1) as a clinically relevant androgen regulated target in prostate cancer. Here we find that TSPAN1 is acutely induced by androgens, and is significantly upregulated in prostate cancer relative to both normal prostate tissue and benign prostate hyperplasia (BPH). We also show for the first time, that TSPAN1 expression in prostate cancer cells controls the expression of key proteins involved in cell migration. Stable upregulation of TSPAN1 in both DU145 and PC3 cells significantly increased cell migration and induced the expression of the mesenchymal markers SLUG and ARF6. Our data suggest TSPAN1 is an androgen-driven contributor to cell survival and motility in prostate cancer.

• Item Type: Articles
• Additional Information: This work was funded by Prostate Cancer UK (PG12-34), The J. G. W Patterson Foundation, Newcastle University Special Trustees, the BBSRC (grants BB/1006923/1 and BB/K018957/1) and the Breast Cancer Now (grant number 2014NovPR355).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Leung, Professor Hing and Rajan, Dr Prabhakar and Elliott, Professor David
• Authors: Munkley, J., McClurg, U. L., Livermore, K. E., Ehrmann, I., Knight, B., Mccullagh, P., Mcgrath, J., Crundwell, M., Harries, L. W., Leung, H. Y., Mills, I. G., Robson, C. N., Rajan, P., and Elliott, D. J.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Scientific Reports
• Publisher: Nature Research
• ISSN: 2045-2322
• ISSN (Online): 2045-2322
• Copyright Holders: Copyright © 2017 The Authors
• First Published: First published in Scientific Reports 7: 5249
• Publisher Policy: Reproduced under a Creative Commons license