Shchepinova, M. M. et al. (2017) MitoNeoD: a mitochondria-targeted superoxide probe. Cell Chemical Biology, 24(10), 1285-1298.e12. (doi: 10.1016/j.chembiol.2017.08.003) (PMID:28890317) (PMCID:PMC6278870)
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Abstract
Mitochondrial superoxide (O2⋅−) underlies much oxidative damage and redox signaling. Fluorescent probes can detect O2⋅−, but are of limited applicability in vivo, while in cells their usefulness is constrained by side reactions and DNA intercalation. To overcome these limitations, we developed a dual-purpose mitochondrial O2⋅− probe, MitoNeoD, which can assess O2⋅− changes in vivo by mass spectrometry and in vitro by fluorescence. MitoNeoD comprises a O2⋅−-sensitive reduced phenanthridinium moiety modified to prevent DNA intercalation, as well as a carbon-deuterium bond to enhance its selectivity for O2⋅− over non-specific oxidation, and a triphenylphosphonium lipophilic cation moiety leading to the rapid accumulation within mitochondria. We demonstrated that MitoNeoD was a versatile and robust probe to assess changes in mitochondrial O2⋅− from isolated mitochondria to animal models, thus offering a way to examine the many roles of mitochondrial O2⋅− production in health and disease.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Caldwell, Dr Stuart and Shchepinova, Maria and Senn, Dr Hans and Cairns, Mr Andrew and Hartley, Professor Richard |
| Authors: | Shchepinova, M. M., Cairns, A. G., Prime, T. A., Logan, A., James, A. M., Hall, A. R., Vidoni, S., Arndt, S., Caldwell, S. T., Prag, H. A., Pell, V. A., Krieg, T., Mulvey, J. F., Yadav, P., Cobley, J. N., Bright, T. P., Senn, H. M., Anderson, R. F., Murphy, M. P., and Hartley, R. |
| College/School: | College of Science and Engineering > School of Chemistry |
| Journal Name: | Cell Chemical Biology |
| Publisher: | Elsevier Cell Press |
| ISSN: | 2451-9456 |
| ISSN (Online): | 2451-9456 |
| Published Online: | 07 September 2017 |
| Copyright Holders: | Copyright © 2017 The Authors |
| First Published: | First published in Cell Chemical Biology 24(10):1285-1298.e12 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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